Insulin-like Growth Factor-I from mouse
SIGMA/I8779 - IGF-I, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym: IGF-I
MDL Number: MFCD00081716
Product Type: Chemical
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| assay | ≥98% (SDS-PAGE and HPLC) |
| biological source | mouse |
| form | lyophilized powder |
| impurities | <0.1 ng/mg (LAL test) |
| mol wt | predicted mol wt ~7.6 kDa |
| packaging | glass bottle of 50 μg (in glass insert) |
| quality | endotoxin tested |
| Quality Level | 100  |
| recombinant | expressed in E. coli |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P05017  |
| Analysis Note: | The biological activty is measured in a serum-free cell proliferation assay using the human breast carcinoma cell line, MCF-7. |
| Application: | Insulin-like Growth Factor-I from mouse has been used: • for the preparation of growth factor cocktail • as a media supplement for mouse embryonic fibroblasts culture • as a media supplement to serve as a positive control to investigate • the effects of Angelica sinensis on myotubes |
| Biochem/physiol Actions: | Insulin-like growth factor I (IGF-I) plays critical roles in cellular energy metabolism and in growth and development, especially prenatal growth. IGF-I is secreted from the liver into circulation in a process regulated by pituitary growth hormone (GH) and so it mediates the growth-promoting activity of GH. After birth IGF-I expression in most extrahepatic tissues declines and hepatic expression of IGF-I becomes GH-regulated. Expression of IGF-I outside the liver is regulated differently, depending on the specific tissues. For example, gonadotropins and sex steroids regulate IGF-I expression within the reproductive system, while parathyroid hormone and sex steroids regulate IGF-I expression in bone. IGF-I is produced in several human tumors. IGF-I is mitogenic for a variety of cells including fibroblasts, osteoblasts, smooth muscle cells, fetal brain cells, neuroglial cells, and erythroid progenitor cells. IGF-I exerts its actions exclusively through the IGF-I receptor (IGF-IR). IGF-I induces endothelial cell migration and is involved in the regulation of angiogenesis. |
| Biochem/physiol Actions: | Potent mitogenic growth factor that mediates the growth-promoting activities of growth hormone postnatally. Mouse and human IGF-I share 97% sequence identity. |
| General description: | Insulin-like growth factor I (IGF-I) belongs to the insulin-like growth factor (IGF; see MIM 147440) family of signaling molecules. IGF-I is also known as somatomedin. In the developing embryo IGF-I is expressed primarily by mesenchymal-derived cells. |
| Packaging: | 50 μg in glass insert |
| Physical form: | Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE and HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |