Anti-Human IgG (Fc specific) antibody produced in goat
SIGMA/I8885 - whole antiserum
MDL Number: MFCD00162436
Product Type: Chemical
ELISA Anti-Human IgG (Fc specific) antibody produced in Goat (Cat. No. I8885), followed by 1:5,000 Anti-Goat IgG (whole molecule)-Peroxidase antibody produced in Rabbit (Cat. No. A5420), was serially diluted to detect Human IgG by indirect ELISA.
| antibody form | whole antiserum |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:50,000 |
| quantitative precipitin assay: 4.0-7.0 mg/mL |
| Application: | Anti-Human IgG (Fc specific) antibody produced in goat has been used in enzyme-linked immunosorbent assay (ELISA). |
| Biochem/physiol Actions: | Immunoglobulin G (IgG) mainly helps in immune defense. It participates in hypersensitivity type II and type III. It provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. |
| Biochem/physiol Actions: | Specificity for the Fc fragment of human IgG is determined by immunoelectrophoresis (IEP) versus normal human serum and Fc fragment of human IgG. No reactivity is observed versus purified human IgA, IgG (Fab fragment), IgM, Bence Jones kappa and Bence Jones lambda myeloma proteins. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Immunoglobulin G (IgG), a widely expressed serum antibody, has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein, usually found as a monomer. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. About 70 % of the total immunoglobulin consists of IgG. |
| Physical form: | Goat Anti-Human IgG is supplied as a liquid containing 0.1% sodium azide (see MSDS) as a preservative. |
| Preparation Note: | For continuous use, store at 2-8°C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. This goat antiserum was maintained at pH 5.0 for 40 minutes to meet U.S.D.A. requirements. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |