Anti-Human IgG (Fab specific) antibody produced in goat
SIGMA/I9010 - whole antiserum
MDL Number: MFCD00162431
Product Type: Chemical
ELISA Anti-Human IgG (Fab specific) antibody produced in Goat (Cat. No. I9010), followed by 1:5,000 Anti-Goat IgG (whole molecule)-Peroxidase antibody produced in Rabbit (Cat. No. A5420), was serially diluted to detect Human IgG by indirect ELISA.
| antibody form | whole antiserum |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:30,000 |
| quantitative precipitin assay: 4.0 mg/mL |
| Application: | Anti-Human IgG (Fab specific) antibody produced in goat has been used in immunological assays. It has also been used in cellulose substrates as immobilized anti-hIgG to detect human immunoglobulin G (hIgG) from fluid matrices. |
| Application: | Anti-Human IgG (Fab specific) antibody produced in goat was used in capture ELISA at a dilution of 1:10,000. |
| Biochem/physiol Actions: | IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. |
| Biochem/physiol Actions: | Reactivity is observed versus purified human IgA, IgM, Bence Jones Kappa and Bence Jones Lambda myeloma proteins. No reactivity is observed versus Fc fragment of human IgG. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Immunoglobulins have two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is usually found as a monomer. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4. About 70 percent of the total immunoglobulin consists of IgG. |
| Physical form: | Supplied as a liquid containing 15 mM sodium azide as a preservative. |
| Preparation Note: | delipidized |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| Hazard Codes | Xn |
| Risk Statements | 22 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |