Interferon β from mouse
SIGMA/I9032 - ≥95%, recombinant, expressed in E. coli, buffered aqueous solution, suitable for cell culture
Synonym: IFN β
MDL Number: MFCD02189136
Product Type: Chemical
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| assay | ≥95% |
| biological source | mouse |
| concentration | 2560000 units/mL |
| form | buffered aqueous solution |
| mol wt | ~20 kDa |
| packaging | pkg of 1 vial |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P01575  |
| Analysis Note: | The biological activity is determined in the cytopathic effect inhibition assay using mouse L929 cells with encephalomyocarditis virus. The cytopathic effect of 50% is produced with approximately 1 unit/ml interferon. The units are determined with respect to NIH international standard reference for mouse interferon β. |
| Application: | Interferon ß from mouse has been used: • to induce the activation of cholesterol 25-hydroxylase, by in vitro stimulation of bone marrow-derived macrophages, dendritic cells and CD11c+ lung macrophages • to stimulate splenocytes, in order to study the secretion and regulation of IFN-γ • to intranasally pre-treat neonatal mice, to study its effects on the protective actions of Lactobacillus rhamnosus GG (LGG) • as a standard to treat MEF-Mx2-luc-BKO reporter cell line for IFN-β measurement, in response to human adenovirus-5 (HAdV-C5) |
| Application: | Interferon β from mouse has been used: • in the stimulation of bone marrow-derived dendritic cells (BMDCs) • as a cytokine to stimulate differentiation of podocytes • to stimulate splenocytes |
| Biochem/physiol Actions: | Cytokine with anti-viral, anti-proliferative, immunoregulatory, and proinflammatory activities. |
| Biochem/physiol Actions: | In presence of virus infection, IFN β (interferon β) is upregulated and plays a crucial role in innate antiviral response. It is also important for anti-inflammatory effects. |
| Biochem/physiol Actions: | Interferon β (IFN-β) suppresses a variety of proinflammatory cytokines in lymphocytes including the interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α). It also favors the synthesis of antagonists associated with interleukin IL-10 and IL-1 receptor. IFN-β downregulates interferon γ production but enhances transforming growth factor β1 expression. It has therapeutic potential in treating rheumatoid arthritis and relapsing-remitting multiple sclerosis (MS). |
| Biochem/physiol Actions: | Interferon β has a role in the activation of human leukocyte antigen (HLA) gene expression. It acts as a messenger during bacterial infections. |
| General description: | IFN β (interferon β) is from the type 1 interferon family of cytokines. |
| General description: | Interferon β (IFN-β) possesses a signal peptide region. It has comparatively more hydrophobic residues and displays 30% homology with IFN-α. |
| General description: | The gene encoding interferon β (Infb1) is localized on mouse chromosome 4. |
| Physical form: | Solution in 20 mM HEPES, pH 6.0, 0.5 M NaCl, 6% glycerol, and 0.1% bovine serum albumin. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% |
| Storage Temp. | −70°C |
| UNSPSC | 12352202 |