Anti-Human IgG (Fc specific) antibody produced in rabbit
SIGMA/I9135 - whole antiserum
Synonym: Human Antiserum; Rabbit Anti Human IgG - Anti-Human IgG (Fc specific) antibody produced in rabbit; Rabbit Anti Human Igg; Rabbit Antiserum
MDL Number: MFCD00162436
Product Type: Chemical
ELISA Anti-Human IgG (Fc specific) antibody produced in Rabbit (Cat. No. I9135), followed by 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545), was serially diluted to detect Human IgG by indirect ELISA.
| antibody form | whole antiserum |
| antibody product type | secondary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:100,000 |
| quantitative precipitin assay: 1.5 mg/mL |
| Application: | Anti-Human IgG (Fc specific) antibody produced in rabbit was used in immunoblotting and immunocytochemistry. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunohistochemistry (1 paper) |
| Biochem/physiol Actions: | IgG activates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a pivotal role in antibody-dependent cell-mediated cytotoxicity (ADCC) and phagocytosis. It might be linked to allergy and has the longest serum half life. |
| Biochem/physiol Actions: | IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. |
| General description: | IgG contains four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, that are associated by inter-chain disulfide bonds. IgG contains four isotypes IgG1, IgG2, IgG3 and IgG4. |
| Preparation Note: | treated to remove lipoproteins |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |