Interleukin-10 from rat
SIGMA/I9154 - >95% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder
Synonym: IL-10
EC Number: 201-064-4
MDL Number: MFCD01635777
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: I9154-25UG.
| assay | >95% (SDS-PAGE) |
| biological source | rat |
| form | lyophilized powder |
| impurities | endotoxin, tested |
| mol wt | predicted mol wt ~19 kDa |
| packaging | pkg of 5 μg |
| potency | 1-35 ng/mL ED50 |
| Quality Level | 100  |
| recombinant | expressed in E. coli |
| storage temp. | −20°C |
| UniProt accession no. | P29456  |
| Analysis Note: | The biological activity is measured in a cell proliferation assay using the murine mast cell line, MC/9. |
| Application: | Interleukin-10 (IL-10) from rat has been used to study the anti-allodynic effect of IL-10 in rat model. |
| Biochem/physiol Actions: | Interleukin-10 is an important regulator of the functions of lymphoid and myeloid cells. IL-10 can block the activation of cytokine synthesis and several accessory functions of macrophages. Human and mouse IL-10 share a 73% sequence homology. However, human IL-10 acts on both human and mouse target cells, while mouse IL-10 has species-specific activity. In mouse, the cellular sources of IL-10 consist of Th2 cells, activated fetal thymocytes, macrophages, keratinocytes, LY-1+ (CD5+), and normal B cells. In human, the cellular sources of IL-10 consist of CD4+ T cells and T cell clones, thymocytes, B cells, B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL-10 stimulates the growth of stem cells, mast cells and thymocytes. IL-10 enhances cytotoxic T cell development, and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing the cell-type dependent expression of either angiogenic or angiostatic factors. |
| Biochem/physiol Actions: | Interleukin-10 regulates lymphoid and myeloid cell functions. It blocks the activation of cytokine synthesis and several accessory functions of macrophages. IL-10 enhances cytotoxic T cell development and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing cell-type dependent expression of either angiogenic or angiostatic factors. |
| Biochem/physiol Actions: | Interleukin-10 regulates lymphoid and myeloid cell functions; blocks the activation of cytokine synthesis and several accessory functions of macrophages; enhances cytotoxic T cell development and co-stimulates B cell differentiation and Ig secretion. |
| Physical form: | Lyophilized from a 0.2 μm filtered in 20 mM Tris, 0.1 M sodium chloride containing 50 μg of bovine serum albumin per 1 μg of cytokine. |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26-36 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Purity | >95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |