Monoclonal Anti-Human IgG1 (Fc specific) antibody produced in mouse
SIGMA/I9388 - clone HP-6001, ascites fluid
MDL Number: MFCD00162492
Product Type: Chemical
ELISA Monoclonal Anti-Human IgG1 (Fc specific) antibody produced in Mouse, Clone: HP-6001 (Cat. No. I9388), followed by 1:750 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917), was serially diluted to detect Human IgG1 by indirect ELISA. Reactivity is observed with Human IgG1k and Human IgG1λ but not with Human IgG2k, Human IgG3k and Human IgG4k.
| antibody form | ascites fluid |
| antibody product type | secondary antibodies |
| biological source | mouse |
| clone | HP-6001, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG2b |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:3,000 |
| Application: | Monoclonal Anti-Human IgG1 (Fc specific) antibody is suitable for use in ELISA and immunoblot. |
| Biochem/physiol Actions: | IgG1 has a molecular weight of 146 kDa and is an abundantly expressed IgG subclass. IgG1 deficiencies contribute to overall decrease in total IgG levels resulting in hypogammaglobulinemia. IgG1 promotes bacterial phagocytosis and mediates vaccine-induced protection from infection. The ratio of IgG2a to IgG1 is crucial for immune response against Leishmania tropica infection. It has high binding affinity towards the Fcγ receptors (FcγR). |
| Biochem/physiol Actions: | Monoclonal Anti-Human IgG1 (Fc specific) is specific for the IgG1 subclass and nonreactive with IgG2, IgG3, and IgG4 in an ELISA. The antibody recognizes an epitope expressed in the Fc region of IgG1. The estimated association constant of this antibody with its ligand is 1x107 L/M. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders Monoclonal Anti-Human IgG1 (Fc specific) antibody is specific for an epitope in the Fc region of human IgG1 and does not detect IgG2, IgG3, and IgG4. The association constant of this antibody with its ligand is estimated to be 1x(107) L/M. This product clone has been tested for specificity using various immunoassays in the IUIS/WHO study. |
| Immunogen: | Purified human IgG myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads |
| Physical form: | Monoclonal Anti-Human IgG1 (Fc specific) is provided as ascites fluid with 15 mM sodium azide. |
| Preparation Note: | Store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |