3-Methylbutanol
SIGMA/I9392 - BioReagent, ≥98.5%, Molecular Biology
Synonym: 3-Methyl-1-butanol; Isoamyl alcohol; Isopentyl alcohol
CAS Number: 123-51-3
Empirical Formula (Hill Notation): C5H12O
Molecular Weight: 88.15
EC Number: 204-633-5
MDL Number: MFCD00002934
Linear Formula: (CH3)2CHCH2CH2OH
Product Type: Chemical
| assay | ≥98.5% |
| density | 0.809 g/mL at 20 °C (lit.) |
| form | liquid |
| grade | Molecular Biology |
| InChI | 1S/C5H12O/c1-5(2)3-4-6/h5 |
| InChI key | PHTQWCKDNZKARW-UHFFFAOYSA |
| pH | 5.6 (20 °C, 25 g/L) |
| product line | BioReagent |
| Quality Level | 200 ![]() |
| SMILES string | CC(C)CCO |
| storage temp. | room temp |
| suitability | suitable for nucleic acid purification |
| technique(s) | RNA extraction: suitable |
| Application: | 3-Methylbutanol (isoamyl alcohol) in combination with phenol and chloroform is used in extraction of RNA. It was used in the synthesis of oligo-RNAs using photocaged adenosine molecules. |
| Application: | 3-Methylbutanol has been used as a component of phenol/chloroform/ isoamyl alcohol solution for DNA extraction from blood and from absorbing substrata based forensic samples and biofilms. |
| General description: | 3-methylbutanol (isoamyl alcohol) is a clear, colorless alcohol commonly used as an organic solvent . It can also be used in phenol-chloroform nucleic acid extraction and purification protocols, where it inhibits RNase activity and reduces foaming. Isoamyl alcohol can also be used for the removal of ethidium bromide from DNA purified by CsCl gradient ultracentrifugation. |
| Packaging: | 1 L in glass bottle |
| Packaging: | 25, 500 mL in glass bottle |
| Symbol | ![]() ![]() GHS02,GHS05,GHS07 |
| Signal word | Danger |
| Hazard statements | H226 - H315 - H318 - H332 - H335 |
| Precautionary statements | P210 - P261 - P280 - P305 + P351 + P338 + P310 - P370 + P378 |
| Hazard Codes | Xn |
| Risk Statements | 10-20-36/37/38-66 |
| Safety Statements | 26-46 |
| RIDADR | UN1105 - class 3 - PG 3 - Pentanols |
| WGK Germany | WGK 1 |
| Flash Point(F) | 109.4 °F - closed cup |
| Flash Point(C) | 43 °C - closed cup |
| Purity | ≥98.5% |
| Density | 0.809 g/mL at 20 °C (lit.) |
| Storage Temp. | room temp |
| UNSPSC | 12352200 |




