2-Propanol
SIGMA/I9516 - BioReagent, ≥99.5%, Molecular Biology
Synonym: sec-Propyl alcohol; IPA; Isopropanol; Isopropyl alcohol
CAS Number: 67-63-0
Empirical Formula (Hill Notation): C3H8O
Molecular Weight: 60.10
EC Number: 200-661-7
MDL Number: MFCD00011674
Linear Formula: (CH3)2CHOH
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: I9516-1L.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: I9516-500ML.
| assay | ≥99.5% |
| autoignition temp. | 750 °F |
| bp | 82 °C (lit.) |
| color | colorless |
| density | 0.785 g/mL at 25 °C (lit.) |
| expl. lim. | 2.0-12.7 %, 93 °C |
| form | liquid |
| grade | Molecular Biology |
| impurities | ≤0.2% water water |
| InChI | 1S/C3H8O/c1-3(2)4/h3-4H,1 |
| InChI key | KFZMGEQAYNKOFK-UHFFFAOYSA |
| mp | −89.5 °C (lit.) |
| product line | BioReagent |
| Quality Level | 200  |
| refractive index | n |
| SMILES string | CC(C)O |
| solubility | water: soluble (completely) |
| storage temp. | room temp |
| suitability | suitable for precipitation of nucleic acids |
| technique(s) | DNA extraction: suitable |
| RNA extraction: suitable | |
| vapor density | 2.1 (vs air) |
| vapor pressure | 33 mmHg ( 20 °C) |
| 44 mmHg ( 25 °C) |
| Application: | 2-Propanol has been used: • in DNA and RNA extraction • for determination of hydroxyproline levels(The Merck Index, 12th ed) • for checking cell viability by MTT assay • in microarray hybridization Suitable for use in DNA precipitation using standard protocols. Also suitable as a solvent for making solutions for molecular biology applications. |
| General description: | Isopropanol is a clear, colorless, polar organic solvent commonly used in chemistry and molecular biology laboratories. It will dissolve a wide range of chemicals, and evaporates quickly. This product (2-propanol) is designated as molecular biology grade and is suitable for the precipitation of nucleic acids. Protocols for the use of isopropanol: • DNA precipitation from mammalian tissue, ethidium bromide extraction from DNA samples, and RNA isolation from cells and tissues. • Protein crystallization. • Isolate retinoids from biological matrices for HPLC and HPLC-MS analysis. |
| Packaging: | 25, 500 mL in glass bottle |
| Symbol |   GHS02,GHS07 |
| Signal word | Danger |
| Hazard statements | H225 - H319 - H336 |
| Precautionary statements | P210 - P233 - P240 - P241 - P242 - P305 + P351 + P338 |
| Hazard Codes | F,Xi |
| Risk Statements | 11-36-67 |
| Safety Statements | 7-16-24/25-26 |
| RIDADR | UN1219 - class 3 - PG 2 - Isopropanol |
| WGK Germany | WGK 1 |
| Flash Point(F) | 53.6 °F - closed cup |
| Flash Point(C) | 12.0 °C - closed cup |
| Purity | ≥99.5% |
| bp | 82 °C (lit.) |
| mp | −89.5 °C (lit.) |
| Density | 0.785 g/mL at 25 °C (lit.) |
| Refractive Index | n |
| Storage Temp. | room temp |
| UNSPSC | 12352104 |