Interleukin-6 from mouse
SIGMA/I9646 - IL-6, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture, carrier free
Synonym: Interleukin-6; mIL-6; mIL-6; IL-6
MDL Number: MFCD01656039
Product Type: Chemical
SDS-PAGE The purity of recombinant Mouse IL-6 (Cat. No. I9646) was determined by SDS-PAGE of reduced IL-6 and staining with Coomassie Blue.
Bioassay for Interleukin 6 The biological activity of recombinant mouse IL-6 (Cat. No. I9646) was measured in a cell proliferation assay using T1165.85.2.1 cells. The EC50 is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay.
| assay | ≥97% (SDS-PAGE) |
| biological source | mouse |
| color | white |
| form | lyophilized powder |
| impurities | ≤1.0EU/mg |
| mol wt | ~21.7 kDa |
| packaging | pkg of 5 and 25 μg |
| potency | 0.02-0.2 ng/mL EC50 |
| quality | endotoxin tested |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P08505  |
| Analysis Note: | The biological activity of recombinant mouse IL-6 was measured in a cell proliferation assay using T1165.85.2.1 cells. |
| Biochem/physiol Actions: | Interleukin-6 (IL-6) is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. IL-6 appears to be directly involved in the responses that occur after infection and injury and may prove to be as important as IL-1 and TNF-α in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. IL-6 induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor (PCT-GF), interferon-β-2 (IFN-β2), monocyte derived human B cell growth factor, B cell stimulating factor (BSF-2), hepatocyte stimulating factor (HSF), Interleukin Hybridoma/Plasmacytoma-1 (IL-HP1). In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 also potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. |
| Physical form: | Lyophilized from a sterile filtered solution with no additives. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥97% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |