Anti-Human IgA (α-chain specific) antibody produced in rabbit
SIGMA/I9889 - IgG fraction of antiserum, buffered aqueous solution
MDL Number: MFCD00162412
Product Type: Chemical
Immunoelectrophoresis Human IgA (upper round well) and Human IgM (bottom round well) were reacted with Anti-Human IgA (α-chain specific) antibody produced in Rabbit (Cat. No. I9889) (canel well) to detect Human IgA through precipitation arcs.
| antibody form | IgG fraction of antiserum |
| antibody product type | secondary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 300  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | : 2.5-5.0 mg/mL using Antibody concentration by precipitin Vs Human serum |
| : 4.0-15.0 mg/mL using Antibody concentration by precipitin Vs Human whey | |
| Ouchterlony double diffusion: 1:32 |
| Application: | Anti-Human IgA (α-chain specific) antibody is suitable for use in: • enzyme-linked immunosorbent assay (ELISA) • quantitative precipitin assay (2.5-5 mg/mL) • immunoelectrophoresis • Ouchterlony double diffusion (1:16) |
| Biochem/physiol Actions: | IgA is a secretory glycoprotein antibody that regulates the physiological balance between commensal intestinal bacteria and the defenses of the host immune system. It prevents the commensal microorganisms from invading the intestines. |
| Biochem/physiol Actions: | Immunoglobulin A (IgA) mediates antibody-dependent cell-mediated cytotoxicity (ADCC) by binding to leukocytes and enhances microbial destruction. It acts as the first line of defense in antigen clearance. IgA initiates the alternative pathway of the complement system to perform protective functions. It reduces inflammation stimulated by the classical pathway complement activation. IgA prevents proteolysis and inhibits the uptake of bacterial, viral or antigen attachment. |
| Biochem/physiol Actions: | The antibody preparation is specific for human IgA when tested against purified human IgA, IgG, IgM, Bence Jones kappa and lambda myeloma proteins. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgA is the highest generated antibody isotype. IgAs are mainly secreted in the intestinal mucosa. Anti-Human IgA (α-chain specific) antibody is specific for the α-chain of IgA in humans. The product reacts with human IgA when tested against purified human IgA, IgG, IgM, and Bence Jones κ and lambda myeloma proteins. |
| General description: | Immunoglobulin A (IgA) exists in two isotypic forms: IgA1 and IgA2. It is produced as secretory IgA (sIgA) and serum IgA. sIgA exists in a monomeric form with two heavy and two light chains. Serum IgA exists in the dimeric form linked by disulfide bind with an additional J chain. IgA is found in saliva, serum, colostrum and nasal fluid. |
| Immunogen: | IgA purified from normal human colostrum |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |