Mouse Monoclonal Antibody Isotyping Reagents
SIGMA/ISO2 - sufficient for 1000 tests (clones) (by ELISA), sufficient for 40 tests (clones) (by immunodiffusion, ODD)
Synonym: Mouse Antibody Isotyping Kit
MDL Number: MFCD08705607
Product Type: Chemical
ELISA ELISA plate coated with six antibodies with different isotypes. Kit components isotype-specific antibodies, followed by 1:5,000 Anti-Goat IgG (whole molecule)-Peroxidase antibody produced in Rabbit (Cat. No. A5420), were diluted to detect tested antibodies isotyping.
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| usage | sufficient for 1000 tests (clones) (by ELISA) |
| sufficient for 40 tests (clones) (by immunodiffusion, ODD) |
| Application: | Determination of the subclass of a monoclonal antibody is helpful for characterization of the antibody, for choosing detection reagents, and for deciding on a purification scheme. |
| Application: | Mouse Monoclonal Antibody Isotyping Reagents has been used in monoclonal antibody isotype determination using enzyme-linked immunosorbent assay (ELISA). It may be used in Ouchterlony immunodiffusion assays. |
| Biochem/physiol Actions: | ISO-2 contains 0.2 mL vials of subclass specific antibodies to all four mouse IgG subclasses, IgA, and IgM. Directions are included for using these reagents in ELISA and Ouchterlony assays to determine the subclass of monoclonal antibodies in ascites, culture supernatants, or purified preparations. Additional reagents may be required, such as conjugates for ELISA assays or agarose and buffer components for Ouchterlony assays. |
| Features and Benefits: | • May be used in a variety of assay formats • Suitable for all antibody forms • Determines all mouse IgG subclasses, IgA, and IgM |
| General description: | The Mouse Monoclonal Antibody Isotyping Reagents enable qualitative isotype determination of mouse monoclonal antibosies derived from hybridoma supernatant, ascites fluid or purified forms. |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |
| Components | goat antisera to mouse IgA .2 mL; goat antisera to mouse IgG3 .2 mL; goat antisera to mouse IgG1 .2 mL; goat antisera to mouse IgG2a .2 mL; goat antisera to mouse IgG2b .2 mL; goat antisera to mouse IgM .2 mL |