Anti-phospho-JNK1/2 (pThr183/pTyr185) antibody produced in rabbit
SIGMA/J4644 - affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-phospho-SAPK (pTpY183/185); Anti-phospho-Stress Activated Protein Kinase (pTpY183/185); Anti-phospho-c-Jun N-terminal Kinase 1/2 (pTpY183/185)
MDL Number: MFCD03455752
Product Type: Chemical
This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous glycerol solution |
| Quality Level | 200  |
| shipped in | wet ice |
| species reactivity | rat, human |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pThr183/pTyr185) |
| technique(s) | immunocytochemistry: 1:250 |
| immunofluorescence: 1:250 | |
| immunohistochemistry: Assay Dependent | |
| western blot: 1:200-1:2000 (using human 293 cells treated with UV radiation) | |
| UniProt accession no. | P45983  |
| Application: | Anti-phospho-JNK1/2 (pThr183/pTyr185 ) antibody concentration is formulated to be used at the recommended dilutions by application. |
| Biochem/physiol Actions: | JNK (c-Jun N-terminal kinase) is phosphorylated by MAPK (mitogen activated protein kinase) kinases 4 and 7 at Thr183 and Tyr185, leading to its activation. It is however not clear as to which MAPKK phosphorylates which amino acid residue. The activation of JNK is highly regulated and is in response to the stress signals that the cell faces. JNK phosphorylates c-Jun, the most important member of the AP-1 transcription factor family that is involved in survival, apoptosis, proliferation and tumorigenesis. JNK also activates other transcription factors such as c-myc and p53. JNK is involved in the phosphorylation of proteins such as Bad, Bcl2 and Bim that result in the execution of apoptosis by release of cytochrome c from mitochondria. Anti-phospho-JNK1/2 (SAPK) (pThr183/pTyr185 ) specifically recognizes the endogenous, active forms of JNK1 and 2 phosphorylated at threonine 183 and tyrosine 185. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | c-Jun N-terminal kinase (JNK, SAPK) is an extensively studied memebr of the MAPK family of kinases and is critical in mediating the apoptotic signaling. There are three isoforms of JNK, JNK 1, 2 and 3. JNK 1 and 2 are ubiquitously expressed; JNK 3 is restricted to cardiac and neuronal tissues. |
| Immunogen: | synthetic peptide derived from a region of JNK1 & 2 that contains threonine183 and tyrosine185. |
| Physical form: | Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, with 50% glycerol, 1% bovine serum albumin (BSA) (IgG and preservatives free) and 0.05% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |