Keratin from human epidermis
SIGMA/K0253 - aqueous solution
CAS Number: 68238-35-7
MDL Number: MFCD00131434
Product Type: Chemical
Cytokeratin intermediate fibers are typically composed of parallel heterodimeric pairs of Type I and Type II peptides. These α-helical dimers are assembled into antiparallel tetramers which form protofibrils composed of four tetramers. Intermediate fibers typically contain ~ eight protofibrils.
In epithelial cells, keratin intermediate filaments are a cytoskeletal component of desmosome cellular junctions.
| assay | ≥90% (SDS-PAGE) |
| biological source | human epidermis |
| form | urea solution |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| technique(s) | ELISA: suitable |
| UniProt accession no. | P04264  |
| Application: | Keratin from human epidermis has been used: • as an analyte and a cross-reactant in the antibody-immobilized chip for testing selectivity coefficients • as a standard in extracellular matrix (ECM) protein in in vitro protein degradation assays. • asa a standard in enzyme linked immunosorbent assay (ELISA) |
| Biochem/physiol Actions: | Keratins are important for the mechanical stability and integrity of epithelial cells and tissues. Some keratins also have regulatory functions and are involved in intracellular signaling pathways, e.g. protection from stress, wound healing, and apoptosis. This diverse group of cytoskeletal scaffolding proteins form intermediate filament networks which provide structural support to keratinocytes that maintain the integrity of the skin. Expression of keratin genes is usually regulated by differentiation of the epidermal cells within the stratifying squamous epithelium. |
| General description: | Keratins are characteristic intermediate filament proteins of the epithelia. In human, keratin is classified into type I and type II. About 54 genes encodes for the keratin protein. The keratin 1 (KRT1) gene is mapped to human chromosome 12q13.13. It is the primary component of hair, skin and nails. |
| Physical form: | Solution in 8 M urea, 50 mM Tris, 0.1 M β-mercaptoethanol and 0.1% sodium azide, pH 8.4 |
| Hazard statements | H412 |
| Precautionary statements | P273 |
| Hazard Codes | Xn |
| Risk Statements | 22-52/53 |
| Safety Statements | 61 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |