Anti-Potassium Channel K2P4.1 (TRAAK) antibody produced in rabbit
SIGMA/K0265 - affinity isolated antibody, lyophilized powder
Synonym: Anti-KCNK4; Anti-TWIK-related arachidonic acid-stimulated K+ channel
MDL Number: MFCD09753858
Product Type: Chemical
Immunohistochemistry Anti-K2P4.1 (TRAAK): Cat. No. K0265: Immunohistochemical staining of Rat cerebellum using Anti-K2P4.1 (TRRAK) antibody. A. K2P4.1 channel appears in Purkinje neuronal processes (red). B. Staining of Purkinje nerve cells with Mouse Anti-Calbindin D28K (a calcium binding protein, green). C. Confocal merge of K2P4.1 channel and Calbindin D28K demonstrates the co-localization of these proteins.
Immunoblotting Anti-K2P4.1 (TRAAK): Cat. No. K0265: Western blot analysis of HEK-293-K2P4.1 transfected cells. Lanes 1. Anti-K2P4.1 (TRAAK) antibody, (1:200). 2. Anti-K2P4.1 (TRAAK) antibody, preincubated with the control peptide antigen.
Immunoblotting Anti-K2P4.1 (TRAAK): Cat. No. K0265: Western blot analysis of Rat cerebellum lysate. Lanes 1. Anti-K2P4.1 (TRAAK) antibody, (1:200). 2. Anti-K2P4.1 (TRAAK) antibody, preincubated with the control peptide antigen.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | lyophilized powder |
| Quality Level | 200  |
| species reactivity | human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: 1:200 using HEK-K2P4.1 transfected cells or rat cerebellum lysate | |
| UniProt accession no. | Q9NYG8  |
| Biochem/physiol Actions: | The gene KCNK4 (potassium two pore domain channel subfamily K member 4) encodes a potassium channel that is mechanosensitive and is regulated by polyunsaturated fatty acids and temperature. The structure and chemical composition of the lipid bilayer has a profound effect on the functioning of this K+ channel. It is found to regulate the noxious input threshold in dorsal root ganglia neurons. |
| Biochem/physiol Actions: | The K2P4.1 antibody is directed against a highly conserved epitope in the intraracellular C-terminal region of the human K2P4.1 channel. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The gene K2P4.1, also referred to as KCNK4 (potassium two pore domain channel subfamily K member 4), encodes a protein belonging to the subfamily of K2P (two pore domain K+ channel) channels called TRAAK (TWIK-related arachidonic acid-stimulated K+ channel). The TRAAK channels are expressed selectively in the nervous system. TRAAK channel contains two protomers, each with two distinct pore domains forming a two-fold symmetric potassium channel. |
| Immunogen: | peptide NLAFIDESSDTQSERGC, corresponding to amino acid residues 343-359 of human K2P4.1. |
| Physical form: | Lyophilized powder from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.025% sodium azide. |
| Preparation Note: | Reconstitute the lyophilized vial with 50 μL or 200 μL deionized water, depending on package size. Further dilutions should be made using a carrier protein, such as BSA (1-3%). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |