Anti-Potassium Channel K2P1.1 (TWIK-1) antibody produced in rabbit
SIGMA/K0390 - affinity isolated antibody, lyophilized powder
Synonym: Anti-Inward rectifying potassium channel TWIK-1
Product Type: Chemical
Immunohistochemistry Anti-K2P1.1 (TWIK-1) (extracellular): Cat. No. K0390: Immunohistochemical staining of Mouse cerebellum using Anti-K2P1.1 (TWIK-1) (extracellular) antibody. A. K2P1.1 channel appears in glial processes (red) . B. Staining of Purkinje nerve cells with Mouse Anti-Calbindin D28K (a calcium binding protein, green). C. Confocal merge of K2P1.1 channel and Calbindin D28K demonstrates the separate localization of these proteins.
Immunoblotting Anti-K2P1.1 (TWIK-1) (extracellular): Cat. No. K0390: Western blot analysis of Rat brain lysate. Lanes 1. Anti-K2P1.1 (TWIK-1) (extracellular) antibody, (1:200). 2. Anti-K2P1.1 (TWIK-1) (extracellular) antibody, preincubated with the control peptide antigen.
Immunoblotting Anti-K2P1.1 (TWIK-1) (extracellular): Cat. No. K0390: Western blot analysis of HEK-293-K2P1.1 transfected cells. Lanes 1. Anti-K2P1.1 (TWIK-1) (extracellular) antibody, (1:300). 2. Anti-K2P1.1 (TWIK-1) (extracellular) antibody, preincubated with the control peptide antigen.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | lyophilized powder |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: 1:200 using rat brain lysates | |
| western blot: 1:300 using HEK-K2P1.1 transfected cells | |
| UniProt accession no. | O00180  |
| Biochem/physiol Actions: | The gene KCNK1 (potassium two pore domain channel subfamily K member 1) is a double-pore potassium channel that functions in maintaining the renal potassium homeostasis. KCNK channel proteins are involved in cell volume, apoptosis, and proliferation. Alterations in these proteins have been associated with various diseases. Decreased levels of KCNK1 in human cardiac atria have been implicated in chronic atrial fibrillation. It functions as a suppressor of anchorage-independent growth in fibroblast. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | K2P1.1 (also named TWIK-1 or KCNK1) is a member of the 2-pore (2P) domain K+ channels family that at the moment includes 14 members. These channels show little time or voltage dependence and are considered to be "leak" or "background" K+ channels, thereby generating background currents, which help set the membrane resting potential and cell excitation. |
| General description: | The gene K2P1.1 (two pore domain K+ channel), also referred to as KCNK1 (potassium two pore domain channel subfamily K member 1), encodes a protein that spans a length of 337-amino acids. It contains four transmembrane regions and the characteristic sequence of potassium channels in the two pore regions. The protein is found to be exclusively expressed in distal tubule. The gene is mapped to human chromosome 1q42.2. |
| Immunogen: | synthetic peptide RQELRKLKRRFLEEHEC, corresponding to amino acid residues 53-69 of human K2P1.1 (KCNK1). |
| Physical form: | Lyophilized from pphosphate buffered saline, pH 7.4, containing 1% BSA and 0.025% sodium azide. |
| Preparation Note: | Reconstitute the lyophilized vial with 50 μL or 200 μL deionized water, depending on package size. Further dilutions should be made using a carrier protein such as BSA (1-3%). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |