Anti-Human Kappa Light Chain (Bound and Free) antibody, Mouse monoclonal
SIGMA/K4377 - clone KP-53, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Human Kappa Light Chain (Bound and Free)
MDL Number: MFCD00162517
Product Type: Chemical
ELISA Monoclonal Anti-Human Kappa Light Chain (Bound and Free) antibody produced in Mouse, Clone: KP-53 (Cat. No. K4377), followed by 1:500 Anti-Mouse IgG (Fc specific)-Peroxidase antibody produced in Goat (Cat. No. A0168), was serially diluted to detect Human Kappa Light Chain (Bound and Free) by indirect ELISA. Reactivity is observed with B-J Kappa, HuIgG and HuIgG2 K but not with B-J Lambda and HuIgG2 λ.
| antibody form | purified from hybridoma cell culture |
| antibody product type | secondary antibodies |
| biological source | mouse |
| clone | KP-53 |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:10,000 |
| Application: | Monoclonal Anti-Human Kappa Light Chain (Bound and Free) antibody produced in mouse has been used for: • immunodetection • in western blot analysis • in intracellular kappa light chain staining for flow cytometric analysis |
| Biochem/physiol Actions: | The antibody is specific for human kappa (κ) light chains. This antibody is reactive with intact IgG (κ), IgM (κ), free kappa light chains, and Bence-Jones kappa light chains. It is non-reactive with lambda light chains. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Human Kappa Light Chain (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins G are made up of two identical light chains and two identical heavy chains, that are covalently connected by interchain disulphide bonds. Genes of kappa and lambda constant region are mapped on to human chromosome 2. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| Preparation Note: | For continuous use, store at 2-8 °C. For extendedstorage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |