KiCqStart® SYBR® Green qPCR ReadyMix™
SIGMA/KCQS00 - For Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems
Synonym: qPCR master mix; sybr green qPCR
Product Type: Chemical
Target gene was amplified from log-fold dilutions of total HeLa cell cDNA (100 ng to 10 pg) using KiCqStart® SYBR® Green qPCR ReadyMix™ or Competitor Q according to each manufacturer′s protocol. Plots represent averages of quadruplicate reactions.
Fast-cycling KiCqStart® SYBR® Green qPCR ReadyMix™ demonstrates equal performance to conventional SYBR Green protocol and reagents.
Representative kit contents
| color | colorless |
| compatibility | for use with Bio-Rad CFX384 |
| for use with Bio-Rad CFX96 | |
| for use with Bio-Rad MiniOpticon | |
| for use with Bio-Rad MJ Chromo4 | |
| for use with Bio-Rad MJ Opticon 2 | |
| for use with Bio-Rad MJ Opticon Cepheid SmartCycler | |
| for use with Bio-Rad MyiQ | |
| for use with Eppendorf® Mastercycler ep realplex | |
| for use with Eppendorf® Mastercycler ep realplex2 s | |
| for use with Illumina Eco qPCR | |
| for use with Qiagen Corbett Rotor-Gene 3000 | |
| for use with Qiagen Corbett Rotor-Gene 6000 | |
| for use with Qiagen Corbett Rotor-Gene Q | |
| for use with Roche LightCycler 480 | |
| detection method | SYBR® Green |
| feature | dNTPs included |
| hotstart | |
| form | liquid |
| input | purified DNA |
| shipped in | dry ice |
| storage condition | protect from light |
| storage temp. | −20°C |
| technique(s) | qPCR: suitable |
| usage | sufficient for 1250 reactions |
| sufficient for 250 reactions | |
| sufficient for 5000 reactions |
| Application: | KiCqStart® SYBR® Green qPCR ReadyMix™ has been used to perform quantitative real-time PCR (qPCR) for the amplification and quantification of genomic DNA extracted from human gut bacterium Prevotella copri. |
| Application: | PCR applications: • Gene expression • DNA quantification • CHiP |
| Features and Benefits: | • Assay results in as little as 33 minutes • Highly efficient and sensitive real-time PCR results • Little/no optimization required |
| General description: | KiCqStart SYBR Green qPCR ReadyMix is a 2X concentrated, ready-to-use master mix that contains all components, except primers and template, for real-time quantitative PCR (qPCR) This unique combination of proprietary buffer, stabilizers, and Hot-Start Taq DNA polymerase delivers maximum PCR efficiency, sensitivity, specificity and robust fluorescent signal using fast, or conventional, cycling protocols with SYBR Green qPCR. Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step. |
| Legal Information: | Bio-Rad is a registered trademark of Bio-Rad Laboratories, Inc. |
| Legal Information: | CFX384 is a trademark of Bio-Rad Laboratories, Inc. |
| Legal Information: | CFX96 is a trademark of Bio-Rad Laboratories, Inc. |
| Legal Information: | Chromo4 is a trademark of Bio-Rad Laboratories, Inc. |
| Legal Information: | Eppendorf is a registered trademark of Eppendorf AG |
| Legal Information: | KiCqStart is a registered trademark of QIAGEN Beverly Inc. |
| Legal Information: | LightCycler is a registered trademark of Roche |
| Legal Information: | Mastercycler is a registered trademark of Eppendorf AG |
| Legal Information: | MiniOpticon is a trademark of Bio-Rad Laboratories, Inc. |
| Legal Information: | Opticon is a trademark of Bio-Rad Laboratories, Inc. |
| Legal Information: | ReadyMix is a trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | Rotor-Gene is a registered trademark of Qiagen GmbH |
| Legal Information: | SmartCycler is a registered trademark of Cepheid, Inc. |
| Legal Information: | SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries |
| Other Notes: | 2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), KiCqStart Taq DNA Polymerase, SYBR Green dye, and stabilizers packaging: 250 reactions* = 2 X 1.25 mL tubes 1250 reactions* = 10 X 1.25 mL tubes 5000 reactions* = 1 X 50 mL tube *number of reactions based on a 20uL volume |
| Other Notes: | Instrument Compatibility: Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument. KiCqStart SYBR Green qPCR ReadyMix does not contain an internal reference dye. Compatible instruments include: • Bio-Rad® CFX384™ • Bio-Rad CFX96™ • Bio-Rad MiniOpticon™ • Bio-Rad/MJ Chromo4™ • Bio-Rad/MJ Opticon 2 • Bio-Rad/MJ Opticon™ • Cepheid SmartCycler® • Eppendorf Mastercycler® ep realplex • Eppendorf Mastercycler ep realplex2 s • Illumina Eco qPCR • Qiagen/Corbett Rotor-Gene® 3000 • Qiagen/Corbett Rotor-Gene 6000 • Qiagen/Corbett Rotor-Gene Q • Roche LightCycler® 480 |
| Other Notes: | Storage Conditions: KiCqStart SYBR Green qPCR ReadyMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41106300 |