KiCqStart® SYBR® Green qPCR ReadyMix™
SIGMA/KCQS01 - Low ROX™, for ABI and Stratagene instruments
Synonym: qPCR master mix; qPCR master mix; sybr green qPCR
Product Type: Chemical
Target gene was amplified from log-fold dilutions of total HeLa cell cDNA (100 ng to 10 pg) using KiCqStart® SYBR® Green qPCR ReadyMix™ or Competitor Q according to each manufacturer′s protocol. Plots represent averages of quadruplicate reactions.
Fast-cycling KiCqStart® SYBR® Green qPCR ReadyMix™ demonstrates equal performance to conventional SYBR Green protocol and reagents.
Representative kit contents
| color | colorless |
| compatibility | for use with ABI 7500 |
| for use with ABI 7500 Fast | |
| for use with ABI ViiA 7 | |
| for use with Agilent AriaMx | |
| for use with Douglas Scientific IntelliQube | |
| for use with Qiagen Rotor-Gene Q | |
| for use with QuantStudio™ | |
| for use with Strategene Mx3000P | |
| for use with Strategene Mx3005P | |
| for use with Strategene Mx4000 | |
| detection method | SYBR® Green |
| feature | dNTPs included |
| hotstart | |
| form | liquid |
| input | purified DNA |
| shipped in | dry ice |
| storage condition | protect from light |
| storage temp. | −20°C |
| technique(s) | qPCR: suitable |
| usage | sufficient for 1250 reactions |
| sufficient for 250 reactions | |
| sufficient for 5000 reactions |
| Analysis Note: | Kit components are free of contaminating DNase and RNase. KiCqStart® SYBR® Green qPCR ReadyMix™, Low ROX is functionally tested in qPCR. Kinetic analysis must demonstrate linear resolution over six orders of dynamic range (r2 > 0.995) and a PCR efficiency > 90%. |
| Application: | KiCqStart® SYBR® Green qPCR ReadyMix™ has been used: • for the amplification and quantification of DNA in real-time PCR (qPCR) assay • in the amplification and quantification of transcripts in 2-step quantitative reverse transcription polymerase chain reaction (qRT-PCR) • in the amplification of complementary DNA (cDNA) by real-time PCR (qPCR) assay |
| Application: | PCR applications: • Gene expression • DNA quantification • CHiP |
| Features and Benefits: | • Assay results in as little as 33 minutes • Highly efficient and sensitive real-time PCR results • Little/no optimization required |
| General description: | KiCqStart SYBR Green qPCR ReadyMix is a 2X concentrated, ready-to-use master mix that contains all components, except primers and template, for real-time quantitative PCR (qPCR) This unique combination of proprietary buffer, stabilizers, and Hot-Start Taq DNA polymerase delivers maximum PCR efficiency, sensitivity, specificity and robust fluorescent signal using fast, or conventional, cycling protocols with SYBR Green qPCR. Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step. |
| Legal Information: | KiCqStart is a registered trademark of QIAGEN Beverly Inc. |
| Legal Information: | QuantStudio is a trademark of IROA Technologies LLC |
| Legal Information: | ReadyMix is a trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries |
| Legal Information: | SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries |
| Other Notes: | 2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), KiCqStart Taq DNA Polymerase, SYBR Green dye, ROX Reference Dye (for 580-585 nm excitation), and stabilizers packaging: 250 reactions* = 2 X 1.25 mL tubes 1250 reactions* = 10 X 1.25 mL tubes 5000 reactions* = 1 X 50 mL tube *number of reactions based on a 20uL volume |
| Other Notes: | Storage Conditions: KiCqStart SYBR Green qPCR ReadyMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41106300 |