KiCqStart® SYBR® Green qPCR ReadyMix™
SIGMA/KCQS02 - with ROX™ for ABI instruments
Synonym: qPCR master mix; qPCR master mix; sybr green qPCR
Product Type: Chemical
Target gene was amplified from log-fold dilutions of total HeLa cell cDNA (100 ng to 10 pg) using KiCqStart® SYBR® Green qPCR ReadyMix™ or Competitor Q according to each manufacturer′s protocol. Plots represent averages of quadruplicate reactions.
Fast-cycling KiCqStart® SYBR® Green qPCR ReadyMix™ demonstrates equal performance to conventional SYBR Green protocol and reagents.
Representative kit contents
| color | colorless |
| compatibility | for use with ABI 5700 |
| for use with ABI 7000 | |
| for use with ABI 7300 | |
| for use with ABI 7700 | |
| for use with ABI 7900 | |
| for use with ABI 7900 HT | |
| for use with ABI 7900 HT Fast | |
| for use with ABI StepOne | |
| for use with ABI StepOnePlus | |
| detection method | SYBR® Green |
| feature | dNTPs included |
| hotstart | |
| form | liquid |
| input | purified DNA |
| shipped in | dry ice |
| storage condition | protect from light |
| storage temp. | −20°C |
| technique(s) | qPCR: suitable |
| usage | sufficient for 1250 reactions |
| sufficient for 250 reactions | |
| sufficient for 5000 reactions |
| Application: | KiCqStart® SYBR® Green qPCR ReadyMix™ has been used: • in the amplification and quantification of cDNA reverse transcribed from RNA extracted from mice brain samples in a 2-step RT-qPCR assay • to analyze DNA purified by ChIP technique • to perform gene expression analysis • in amplification of RNA isolated from hearts of adult TL wild-type fish by quantitative real-time polymerase chain reaction (PCR) |
| Application: | PCR applications: • Gene expression • DNA quantification • CHiP |
| Features and Benefits: | • Assay results in as little as 33 minutes • Highly efficient and sensitive real-time PCR results • Little/no optimization required |
| General description: | KiCqStart SYBR Green qPCR ReadyMix is a 2X concentrated, ready-to-use master mix that contains all components, except primers and template, for real-time quantitative PCR (qPCR) This unique combination of proprietary buffer, stabilizers, and Hot-Start Taq DNA polymerase delivers maximum PCR efficiency, sensitivity, specificity and robust fluorescent signal using fast, or conventional, cycling protocols with SYBR Green qPCR. Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step. |
| Legal Information: | KiCqStart is a registered trademark of QIAGEN Beverly Inc. |
| Legal Information: | ReadyMix is a trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries |
| Legal Information: | SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries |
| Other Notes: | 2X reaction buffer containing optimized concentrations of MgCl2, dNTPs, (dATP, dCTP, dGTP, dTTP), KiCqStart Taq DNA Polymerase, SYBR Green dye, ROX Reference Dye (for 580-585 nm excitation), and stabilizers. packaging: 250 reactions* = 2 X 1.25 mL tubes 1250 reactions* = 10 X 1.25 mL tubes 5000 reactions* = 1 X 50 mL tube *number of reactions based on a 20uL volume |
| Other Notes: | Storage Conditions: KiCqStart SYBR Green qPCR ReadyMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41106300 |