KiCqStart® SYBR® Green qPCR ReadyMix™
SIGMA/KCQS03 - iQ™, with fluorescein for Bio-Rad systems
Product Type: Chemical
Target gene was amplified from log-fold dilutions of total HeLa cell cDNA (100 ng to 10 pg) using KiCqStart® SYBR® Green qPCR ReadyMix™ or Competitor Q according to each manufacturer′s protocol. Plots represent averages of quadruplicate reactions.
Fast-cycling KiCqStart® SYBR® Green qPCR ReadyMix™ demonstrates equal performance to conventional SYBR Green protocol and reagents.
Representative kit contents
| color | colorless |
| compatibility | for use with Bio-Rad iCycler iQ |
| for use with Bio-Rad iQ 5 | |
| for use with Bio-Rad MyiQ | |
| detection method | SYBR® Green |
| feature | dNTPs included |
| hotstart | |
| form | liquid |
| input | purified DNA |
| shipped in | dry ice |
| storage condition | protect from light |
| storage temp. | −20°C |
| technique(s) | qPCR: suitable |
| usage | sufficient for 1250 reactions |
| sufficient for 250 reactions | |
| sufficient for 5000 reactions |
| Application: | KiCqStart® SYBR® Green qPCR ReadyMix™ has been used for the qPCR analysis of 16S rRNA. Different real-time PCR systems employ different strategies for the normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument. KiCqStart SYBR Green qPCR ReadyMix, iQ contains fluorescein for experimental plate well factor collection on iCycler iQ real-time detection systems or the MyiQ detection system. |
| Application: | PCR applications: • Gene expression • DNA quantification • CHiP |
| Features and Benefits: | • Assay results in as little as 33 minutes • Highly efficient and sensitive real-time PCR results • Little/no optimization required |
| General description: | KiCqStart SYBR Green qPCR ReadyMix is a 2X concentrated, ready-to-use master mix that contains all components, except primers and template, for real-time quantitative PCR (qPCR) This unique combination of proprietary buffer, stabilizers, and Hot-Start Taq DNA polymerase delivers maximum PCR efficiency, sensitivity, specificity and robust fluorescent signal using fast, or conventional, cycling protocols with SYBR Green qPCR. Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step. |
| Legal Information: | iQ is a trademark of Bio-Rad Laboratories, Inc. |
| Legal Information: | KiCqStart is a registered trademark of QIAGEN Beverly Inc. |
| Legal Information: | ReadyMix is a trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries |
| Other Notes: | 2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), KiCqStart Taq DNA Polymerase, SYBR Green dye, 20 nM fluorescein, and stabilizers packaging: 250 reactions* = 2 X 1.25 mL tubes 1250 reactions* = 10 X 1.25 mL tubes 5000 reactions* = 1 X 50 mL tube *number of reactions based on a 20uL volume |
| Other Notes: | Storage Conditions: KiCqStart SYBR Green qPCR ReadyMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41106300 |