KiCqStart® Probe qPCR ReadyMix™, ROX™
SIGMA/KCQS06 - with ROX for ABI instruments
Synonym: Kicqstart® Probe qPCR ReadyMix™
Product Type: Chemical
KiCqStart Probe qPCR Readymix provides sensitivity across a range of template input amounts. Log-fold dilutions: 20ng to 20pg and NTC triplicate qPCRs for each input quantity.
| color | light blue |
| compatibility | ABI 5700 |
| ABI 7000 | |
| ABI 7300 | |
| ABI 7700 | |
| ABI 7900 | |
| ABI 7900 HT | |
| ABI 7900 HT Fast | |
| ABI StepOne | |
| ABI StepOnePlus | |
| detection method | probe-based |
| feature | dNTPs included |
| hotstart | |
| form | liquid |
| input | purified DNA |
| shipped in | dry ice |
| storage condition | protect from light |
| storage temp. | −20°C |
| technique(s) | qPCR: suitable |
| usage | sufficient for 1250 reactions |
| sufficient for 250 reactions | |
| sufficient for 5000 reactions |
| Application: | KiCqStart® Probe qPCR ReadyMix™, ROX™ has been used for amplification of cDNA of a stemness markers genes by quantitative reverse transcription-polymerase chain reaction (RT-qPCR). Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart One-Step Probe RT-qPCR ReadyMix does not contain an internal reference dye. |
| Features and Benefits: | • Assay results in as little as 33 minutes • Highly efficient and sensitive real-time PCR results • Little/no optimization required |
| General description: | KiCqStart® Probe qPCR ReadyMix™ is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. KiCqStart Probe qPCR ReadyMix is provided as a 2X concentrated ready-to-use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue color of an inert tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product. A key component of KiCqStart Probe qPCR ReadyMix is an ultra-pure, processive thermostable DNA polymerase that is free of detectable E. coli DNA§. This PCR master mix is ideal for demanding qPCR applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy samples. The enzyme in KiCqStart Probe qPCR ReadyMix is combined with high avidity monoclonal antibodies to provide a stringent automatic hot-start that allows reaction assembly, and temporary storage, at room temperature prior to PCR amplification. |
| Legal Information: | KiCqStart is a registered trademark of QIAGEN Beverly Inc. |
| Legal Information: | ReadyMix is a trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries |
| Legal Information: | TaqMan is a registered trademark of Roche Molecular Systems, Inc. |
| Other Notes: | 2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, ROX reference dye, inert blue qPCR dye, and stabilizers |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41106300 |