KiCqStart® One-Step Probe RT-qPCR ReadyMix™, Low ROX™
SIGMA/KCQS08 - with Low ROX for ABI and Stratagene instruments
Synonym: KiCqStart® One Step Probe RT-qPCR Readymix™
Product Type: Chemical
KiCqStart One-Step Probe RT-qPCR Readymix supports reproducible gene amplification across a wide dynamic range.
KiCqStart One-Step Probe RT-qPCR ReadyMix is highly resistant to common sample-based inhibitors.
| color | light blue |
| compatibility | ABI 7500 |
| ABI 7500 Fast | |
| ABI QuantStudio | |
| ABI ViiA 7 | |
| Agilent AriaMx | |
| Douglas Scientific IntelliQube | |
| Qiagen Corbett Rotor-Gene Q | |
| Strategene Mx3000P | |
| Strategene Mx3005P | |
| Strategene Mx4000 | |
| detection method | probe-based |
| feature | dNTPs included |
| hotstart | |
| form | liquid |
| input | purified RNA |
| shipped in | dry ice |
| storage condition | protect from light |
| storage temp. | −20°C |
| technique(s) | qPCR: suitable |
| usage | sufficient for 500 reactions |
| Application: | Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart® One-Step Probe RT-qPCR ReadyMix™ does not contain an internal reference dye. Compatible instruments include: • Applied Biosystems® 7500 • Applied Biosystems 7500 Fast • Applied Biosystems ViiA 7 • Applied Biosystems QuantStudio • Agilent® AriaMx • Douglas Scientific IntelliQube • QIAGEN® Rotor-Gene® Q • Stratagene Mx3000P® • Stratagene Mx3005P® • Stratagene Mx4000™ |
| Features and Benefits: | • Assay results in as little as 33 minutes • Highly efficient and sensitive real-time PCR results • Little/no optimization required |
| General description: | KiCqStart® One-Step Probe RT-qPCR ReadyMix™ is a ready-to-use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as TaqMan® 5’-hydrolysis probes on real-time PCR systems (refer to instrument compatibility section to select the appropriate reagent for your machine). First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets as well as high throughput gene-expression studies. The system has been optimized to deliver maximum RT-PCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. KiCqStart® One-Step Probe RT-qPCR ReadyMix™ contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries. The reverse transcriptase is an engineered M-MLV with reduced RNase H activity and improved activity and stability at higher temperatures. The use of higher temperatures (50 to 55°C) for the first-strand step of one-step RT-qPCR provides higher specificity for primer annealing and disruption of RNA secondary structure that can interfere with cDNA synthesis. KiCqStart® One-Step Probe RT-qPCR ReadyMix™ is highly resistant to PCR inhibitors. A key component of the ReadyMix™ is an ultra-pure, highly processive, thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This provides an extremely stringent automatic hot-start that minimizes the potential for primer-dimer and other non-specific PCR artifacts. The light blue color of the inert dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. The dye does not interfere with qPCR performance or affect the stability of the product. |
| Legal Information: | Agilent is a registered trademark of Agilent Technologies, Inc. |
| Legal Information: | Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries |
| Legal Information: | KiCqStart is a registered trademark of QIAGEN Beverly Inc. |
| Legal Information: | Mx3000P is a registered trademark of Agilent Technologies, Inc. |
| Legal Information: | Mx3005P is a registered trademark of Agilent Technologies, Inc. |
| Legal Information: | Mx4000 is a trademark of Agilent Technologies, Inc. |
| Legal Information: | Qiagen is a registered trademark of Qiagen Group |
| Legal Information: | ReadyMix is a trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | Rotor-Gene is a registered trademark of Qiagen GmbH |
| Legal Information: | ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries |
| Legal Information: | TaqMan is a registered trademark of Roche Molecular Systems, Inc. |
| Other Notes: | 2X reaction buffer containing dATP, dCTP, dGTP, dTTP, magnesium chloride, reverse transcriptase, RNase inhibitor protein, hot-start DNA polymerase, ROX reference dye, inert blue qPCR dye, and stabilizers packaging: 500 reactions* = 5 X 1 mL tubes *number of reactions based on a 20uL volume |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41106300 |