Anti-Luciferase antibody produced in rabbit
SIGMA/L0159 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti Luciferase Antibody; Firefly Luciferase Antibody; Luciferase Antibody; Luciferase Antibody - Anti-Luciferase antibody produced in rabbit
MDL Number: MFCD01092407
Product Type: Chemical
Immunofluorescence HEK-293T cells overexpressing luciferase were fixed and stained 10 μg/mL Anti-Luciferase (Cat. No. l0159). The antibody was developed using Goat Anti-Rabbit IgG, FITC conjugate.
Immunoblotting Lysates of HEK-293T cells overexpressing luciferase was separated on SDS-PAGE and probed with Anti-Luciferase (Cat. No. L0159). The antibody was developed using Goat Anti-Rabbit IgG-Alkaline Phosphatase (Cat. No. A9919) and a colorimetric substrate. Lanes 1. Antibody dilution 1:1,000 2. Antibody dilution 1:1,000 + luciferase immunizing peptide
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 1:500-1:1,000 using eukaryotic cells transfected with a plasmid bearing the luciferase gene |
| western blot: 1:10,000 using purified Luciferase |
| Application: | Anti-Luciferase antibody produced in rabbit has been used in immunoblotting and immunohistochemistry. |
| Biochem/physiol Actions: | The antibody identifies recombinant luciferase in eukaryotic cells transfected with a plasmid bearing the luciferase gene. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Analysis of gene expression is commonly assayed by transient transfection. These systems are usually based on the use of fusion genes which are inserted into cells, and expression of the gene is assayed within 48 hours after introduction of DNA. Usually the fusion consists of the promoter binding site or enhancer sequence under study which is attached to a reporter gene. The amount of the reporter protein synthesized under the experimental conditions is presumed to reflect the ability of the sequences studied to direct or promote transcription. Luciferase has become one of the more widely used reporter enzymes. The reporter plasmid contains the gene from the firefly Photinus pyralis. The enzyme catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg+2 and ATP. Mixing these reagents with the cell extract containing luciferase results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. The luciferase assay is fast and sensitive and does not require a radioactive substrate as in the CAT assay. A disadvantage of the luciferase assay is that it requires a rather expensive instrument, the luminometer, to measure enzyme activity. |
| Immunogen: | firefly (Photinus pyralis) luciferase. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |