Anti-LAMP1−Cy3™ antibody produced in rabbit
SIGMA/L0419 - 1-2 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-CD107a; Anti-LAMPA; Anti-LGP120; Anti-
MDL Number: MFCD01864611
Product Type: Chemical
Immunofluorescence Co-staining of peroxisomes and lysosomes in HeLa cells. HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.4% saponin. Fixed cells were co-stained with 20 μg/mL Rabbit Anti-PMP70, Atto 488 Conjugate (Cat. No. P0090) (peroxisomes stained in green) and 2 μg/mL Rabbit Anti-LAMP1, Cy3 Conjugate (Cat. No. L0419) (lysosomes stained in red). Cells were counterstained with DAPI (blue) to stain nuclei.
Direct Immunofluorescence Lysosomal staining in NIH-3T3 cells. NIH-3T3 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.1% saponin. Fixed cells were stained with 2 μg/mL Rabbit Anti-LAMP1, CY3 Conjugate (Cat. No. L0419).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1-2 mg/mL |
| conjugate | CY3 conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct immunofluorescence: 1-2 μg/mL using human HeLa cells, rat NRK cells and mouse 3T3 cells |
| UniProt accession no. | P11279  |
| Application: | Anti-LAMP1-Cy3™ antibody produced in rabbit has been used in immunofluorescence. |
| Biochem/physiol Actions: | The gene LAMP1 (lysosomal associated membrane protein 1) encodes a membrane glycoprotein that functions as an intracellular receptor. It is found to be expressed in the cytoplasm of several types of tumor cells and may be involved in tumor invasion. Lamp1 is crucial for perforin trafficking to the lytic granules and motility of these lytic granules. Its knockdown leads to inhibition of cytotoxicity of human natural killer cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Lysosomal-associated membrane protein 1 (LAMP1), also termed LGP120, is a heavily glycosylated lysosomal membrane protein with a molecular mass of 120 kDa. It consists of a 40 kDa core polypeptide with O-linked and 18 asparagine-linked oligosaccharide side chains. LAMP1 protein contains a leader sequence, a large intralumenal region consisting of 2 homologous domains separated by a hinge region rich in proline and serine, a 24-amino acid transmembrane region, and a short cytoplasmic tail containing the lysosomal membrane targeting signal. |
| General description: | The gene LAMP1 (lysosomal-associated membrane protein 1) encodes a type I transmembrane protein that has a short cytoplasmic tail containing a lysosome-targeting signal of GYQTI(382)-COOH. The gene is mapped to human chromosome 13q34. |
| Legal Information: | Cy3 is a trademark of Cytiva |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |