Lectin from Pisum sativum (pea)
SIGMA/L0770 - FITC conjugate, lyophilized powder
Synonym: Pisum sativum agglutinin; PSA
Product Type: Chemical
Agglutination assay: Lectin from Pisum sativum (pea) (Cat. No. L0770) was serially diluted (2-fold, top to bottom of plate) and incubated with human erythrocytes type: O (4% v/v RBC) to calculate Minimum agglutination titer (MAT). Agglutination is defined as the inability of cells to form a tight button. PBS was used as blank.
| composition | Protein, ≥95% biuret |
| conjugate | FITC conjugate |
| extent of labeling | 2-4 mol FITC per mol protein (Lowry). |
| form | lyophilized powder |
| impurities | salt, free |
| potency | <20 μg per mL agglutination activity |
| Quality Level | 200  |
| storage temp. | −20°C |
| Analysis Note: | Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C. |
| Application: | Lectin from Pisum sativum (pea) has been used to determine acrosomal status and viability of spermatozoa and to assess sperm acrosome reaction (AR) patterns |
| Biochem/physiol Actions: | Lectin from Pisum sativum (pea) possess anti-hepatoma effect and mitogenicity to lymphocytes, like concanavalin A (Con A). |
| Biochem/physiol Actions: | PSA is not blood group specific, but has an affinity for terminal α- |
| General description: | Lectin from Pisum sativum (pea) is a tetrameric glycoprotein of about 48 kDa. It is isolated from peas. Pisum sativum agglutinin is specific for α- |
| Packaging: | 2 mg in serum bottle |
| Preparation Note: | Conjugates are prepared from affinity purified lectin. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P280 - P342 + P311 |
| Hazard Codes | Xn |
| Risk Statements | 42/43 |
| Safety Statements | 36 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |