Anti-Lamin A (C-terminal) antibody produced in rabbit
SIGMA/L1293 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-LAMA; Anti-LMN1; Anti-LMNA; Anti-Lamin A/C
MDL Number: MFCD01633588
Product Type: Chemical
Western Blotting JOURNAL CITATION: Crude subcellular fractionation of cultured mammalian cell lines. By: Holden, P. & Horton, W. A. in BMC Res Notes, 2009. PubMed ID: 20003239 Image collected and cropped by CiteAb from the following publication, (http://bmcresnotes.biomedcentral.com/articles/10.1186/1756-0500-2-243), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence
HeLa cells were fixed and permeabilized with 3% paraformaldehyde followed by 0.5% Triton-X-100. Fixed cells were stained with 1 μg/mL Anti-Lamin A (C-terminal) (Cat. No. L1293) and developed with Goat Anti-Rabbit IgG, Cy3 conjugate.
HeLa cells were fixed and permeabilized with 3% paraformaldehyde followed by 0.5% Triton-X-100. Fixed cells were stained with 1 μg/mL Anti-Lamin A (C-terminal) (Cat. No. L1293) and developed with Goat Anti-Rabbit IgG, Cy3 conjugate.
Enhanced Validation-By Independent Antibodies HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton. Fixed cells were stained with 0.5 μg/mL Anti-Lamin A, mature antibody, Mouse monoclonal, Clone: 4A4 (Cat. No. SAB4200420) (A) and developed using Goat Anti-Mouse IgG, Cy3 conjugate, and 1 μg/mL Anti-Lamin A (C-terminal) antibody produced in Rabbit (Cat. No. L1293) (B). The antibody was developed using Goat Anti-Rabbit IgG, FITC conjugate antibody. Results Two Anti-Lamin A antibodies, SAB4200420 (A) and L1293 (B) target different regions of Lamin A show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunofluorescence NIH/3T3 Mouse fibroblast cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 2 μg/mL Anti-Lamin A (C-terminal) antibody produced in Rabbit (Cat. No. L1293). The antibody was developed using Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No.F9887).
Immunofluorescence NRK normal Rat kidney cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 2 μg/mL Anti-Lamin A (C-terminal) antibody produced in Rabbit (Cat. No. L1293). The antibody was developed using Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No.F9887).
Western Blotting HeLa cells nuclear extract were separated on SDS-PAGE and probed with Anti-Lamin A (C-terminal) antibody produced in Rabbit (Cat. No. L1293). The antibody was developed using 1:30,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 0.1 μg/mL antibody 2. 0.2 μg/mL antibody 3. 0.4 μg/mL antibody 4. 0.1 μg/mL antibody + inhibitor 5. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~70 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 1-2 μg/mL using human HeLa, rat NRK, and mouse 3T3 cells |
| western blot: 0.1-0.2 μg/mL using human HeLa nuclear extract | |
| UniProt accession no. | P02545  |
| Application: | Anti-Lamin A (C-terminal) antibody is suitable for use in western blot (0.1-0.2 μg/mL) using HeLa nuclear extract. This antibody can also be used in indirect immunofluorescence (1-2 μg/mL) using HeLa cells, rat NRK and mouse 3T3 cells. Additionally, anti-Lamin A (C-terminal) antibodies are suitable for use in immunoblotting (approx. 70 kDa). Cleaved fragments of lamin A may form additional bands at 45-50 kDa. |
| Application: | Anti-Lamin A (C-terminal) antibody produced in rabbit has been used in: • western blotting • immunofluorescence microscopy • immunohistochemistry |
| Biochem/physiol Actions: | Lamin A cut into a 47kDa fragment that facilitates chromatin condensation and nuclear degradation during cell death |
| Biochem/physiol Actions: | Mutations in lamin A and C have been linked to a variety of rare human diseases including muscular dystrophy, lipodystrophy, cardiomyopathy, neuropathy, and progeroid syndromes (collectively termed laminopathies) and to premature aging (Hutchinson-Gilford progeria syndrome). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Lamin is a structural protein of the nuclear lamina. Lamin A is a type A lamin encoded by the LMNA gene. Lamin A contains 664 amino acids and is expressed in most somatic cells. It contains α-helical rod domain to enable assembly into filaments, a nuclear localization sequence, and a carboxy-terminal CAAX box isoprenylation sequence for nuclear membrane targeting. |
| Immunogen: | synthetic peptide corresponding to amino acids 598-611 of human lamin A with a C-terminal added cysteine, conjugated to KLH. The corresponding sequence differs by one amino acid in rat, three amino acids in mouse, and by a gap of one amino acid in both rat and mouse lamin A. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |