L-Lactic Dehydrogenase from bovine muscle
SIGMA/L1378 - Type X, ammonium sulfate suspension, ≥600 units/mg protein
Synonym: (S)-Lactate: NAD+ oxidoreductase; L-LDH; LAD; LD; Lactate
CAS Number: 9001-60-9
EC Number: 232-617-8
MDL Number: MFCD00131466
Product Type: Chemical
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| application(s) | life science and biopharma |
| assay | 3.0—25.0 mg protein/mL (biuret) |
| biological source | bovine muscle |
| color | white |
| foreign activity | pyruvate kinase ≤0.01% |
| form | ammonium sulfate suspension |
| manufacturer/tradename | Sigma-Aldrich |
| pH | 7.5 |
| Quality Level | 200  |
| specific activity | ≥600 units/mg protein |
| storage temp. | 2-8°C |
| technique(s) | activity assay: suitable |
| type | Type X |
| UniProt accession no. | P19858  |
| Q5E9B1 |
| Analysis Note: | Protein determined by biuret. |
| Application: | L-Lactic Dehydrogenase from bovine muscle has been used in lactate assay to assess lactate tissue accumulation. |
| Biochem/physiol Actions: | Also catalyzes the oxidation of other |
| Biochem/physiol Actions: | Lactate dehydrogenase (LDH) plays a critical role as a checkpoint in gluconeogenesis and DNA metabolism. It plays a vital role in maintaining homeostasis during periods of low oxygen availability. As an oxidoreductase enzyme, lactate dehydrogenase catalyzes the reversible conversion of pyruvate to lactate using NADH. This enzyme is crucial for the anaerobic metabolism of glucose when oxygen is either absent or in short supply. LDH is the sole serum biomarker effective for evaluating metastatic melanomas. |
| General description: | Research Area: Cell Signaling Lactate dehydrogenase (LDH) is a key enzyme in the anaerobic metabolic pathway and falls under the class of oxidoreductases. This enzyme is found in a wide range of organisms, including both plants and animals. It is widely distributed across all tissues. LDH is primarily a cytoplasmic enzyme, with particularly high concentrations in muscle, liver, and kidney tissues. |
| Other Notes: | One unit will reduce 1.0 μmole of pyruvate to |
| Physical form: | Crystalline suspension in 2.4 M (NH4)2SO4 solution, pH 6.0 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | 3.0—25.0 mg protein/mL (biuret) |
| activity | specific activity: ≥600 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.1.1.27 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |