Lysozyme human
SIGMA/L1667 - Lysobac™, recombinant, expressed in rice, lyophilized powder, ≥100,000 units/mg protein
Synonym: Lysobac™ r Lysozyme; Mucopeptide N-
CAS Number: 12671-19-1
MDL Number: MFCD00131557
Product Type: Chemical
Lysozyme specificity: Peptidoglycans are polymers of β-(1-4)-N-Acetyl-D-glucosamine units. Alternating residues are modified to form N-acetylmuramic acid with the addition of lactate to form branching links to the tetrapeptide.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L1667-1G
| application(s) | cell analysis |
| assay | ≥90% (SDS-PAGE) |
| biological source | human |
| form | lyophilized powder |
| mol wt | 16.5 kDa |
| Quality Level | 200  |
| recombinant | expressed in rice |
| shipped in | dry ice |
| specific activity | ≥100,000 units/mg protein |
| storage temp. | −70°C |
| suitability | suitable for cell lysis |
| technique(s) | cell based assay: suitable |
| UniProt accession no. | P61626  |
| Application: | Lysozyme human has been used: • in the interaction studies with polyphenon-60 by calorimetric and spectroscopic techniques • in the drug release assay of dexamethasone (DEX) - carbon nanotube based delivery system • in the surface plasmon resonance (SPR) for measuring its interaction with human angiotensin I-converting enzyme (ACE) |
| Biochem/physiol Actions: | Lysozyme is an antibacterial enzyme, which acts on the peptidoglycan layer at the on the N-acetylgluosamine (NAG) and N-acetylmuramic acid linkage, resulting in the lysis of microbial cells. It is a potential anti-inflammatory factor and also has anti-fungal and anti-viral functionality. In inflammatory bowel disease, lysozyme is highly expressed in colonic tissue. Point mutations in lysozyme is implicated in the pathogenesis of hereditary non-neuropathic renal amyloidosis. |
| Features and Benefits: | Lysobac™ is a breakthrough for bacterial cell lysis and can be used in diagnostic applications, bioprocessing and life science research. It is recombinant human lysozyme produced in an animal-free production system. Animal-free production eliminates the safety risk and inconsistent lot-to-lot performance of hen egg white lysozyme. Lysobac™ has significantly higher bioactivity than hen egg white lysozyme. One gram replaces 4 grams of hen egg white lysozyme. It also delivers gentle cell lysis, unlike mechanical lysis which can cause protein shear and reduce protein yields by up to 20%. Advantages: • Improves Bacterial Fermentation Efficiency • Greater bacterial lysis activity per mg. • 1 gram of delivers more lysis activity than 4 grams of HEWL • Animal-free and consistent leading to improved regulatory efficiency for drug manufacturing • Improved recombinant protein yield compared to mechanical lysis • Reduces cost of goods |
| General description: | Lysozyme is present in tears, saliva and human milk. The human lysozyme expressed in rice grains is an excellent supplemental source of this antimicrobial protein. Lysozyme is mapped to human chromosome 12q15 and corresponds to a molecular weight of 16.5kDa. It is a positively charged and a C-type lysozyme with α and β structural domains. |
| Legal Information: | Lysobac is a trademark of InVitria |
| Legal Information: | rhLysozyme manufactured in the USA by InVitria. |
| Other Notes: | One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C. |
| Other Notes: | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enz |
| Packaging: | 1, 10 g in glass bottle |
| Packaging: | Package size based on protein content |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| activity | specific activity: ≥100,000 units/mg protein |
| Storage Temp. | −70°C |
| Enzyme Commission (EC) Number | 3.2.1.17 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |