Lectin from Phaseolus vulgaris (red kidney bean)
SIGMA/L1668 - Phytohemagglutinin PHA-P, lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl), BioReagent, suitable for cell culture
Synonym: Phaseolus vulgaris agglutinin; PHA
Product Type: Chemical
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| color | white to off-white |
| form | lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl) |
| potency | ≤10 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells) |
| product line | BioReagent |
| Quality Level | 200  |
| solubility | PBS: 5 mg/mL, clear to slightly hazy, colorless to faintly yellow (pH 6.8) |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Analysis Note: | Potency and solubility expressed in mg lectin/ml |
| Application: | Lectin from Phaseolus vulgaris (red kidney bean) has been used: • for the stimulation of cluster of differentiation (CD4+) T cells • for the activation of primary peripheral blood lymphocytes • for the stimulation of peripheral blood mononuclear cells |
| Biochem/physiol Actions: | PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity. PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins. |
| Packaging: | Sold on the basis of lectin content |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |