Lipase from Candida rugosa
SIGMA/L1754 - Type VII, ≥700 unit/mg solid
Synonym: Triacylglycerol acylhydrolase; Triacylglycerol lipase
CAS Number: 9001-62-1
EC Number: 232-619-9
MDL Number: MFCD00131509
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L1754-25G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L1754-100G
| biological source | (from Candida cylindracea Type VII) |
| color | beige |
| white | |
| form | lyophilized |
| InChI | 1S/C11H9N3O2.Na/c15-8-4-5 |
| InChI key | QWZUIMCIEOCSJF-CHHCPSLASA |
| Quality Level | 200  |
| solubility | water: slightly soluble |
| specific activity | ≥700 unit/mg solid |
| storage condition | (Tightly closed. Dry) |
| storage temp. | 2-8°C |
| technique(s) | cell based assay: suitable |
| type | Type VII |
| Application: | Lipase from Candida rugosa has been used: • to synthesize dextran fatty acid esters • to study the effect of lipase on conjugated linoleic acid (CLA1 and CLA2) production in the presence of sunflower oil and castor oil • to prepare pH-imprinted enzyme for lipase-catalyzed transesterification of dextran T-40 with vinyl decanoate |
| Application: | Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel. |
| Biochem/physiol Actions: | Lipases are enzymes and biocatalysts that catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids. They are useful for biotransformation reactions. It possesses broad substrate tolerance and is highly stereo- and regioselective. Lipases mediate lipid transport. Elevated levels of serum lipase are observed in pancreatitis. Lipases are the preferred catalysts for long-chain fatty acids. Lipases can be used in a wide range of solvents, in both aqueous and non-aqueous media, and thus have found use in applications like organic synthesis. |
| Biochem/physiol Actions: | Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate). |
| Biochem/physiol Actions: | Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate). Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids. |
| General description: | Research area: Cell Structure Lipases are found in pancreatic secretions. This class of enzymes contains α and β hydrolase folds. Candida rugosa produces multiple lipase isoenzymes that have 80% sequence homology. |
| General description: | This product contains lactose as a carrier. |
| Other Notes: | One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.2 at 37 °C. (This is equivalent to approx. 10 microliters of CO2 in 30 minutes.) |
| Packaging: | 5, 10, 25, 100 g in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥700 unit/mg solid |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.1.1.3 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |