Lipopolysaccharides from Escherichia coli K-235
SIGMA/L2018 - purified by gel-filtration chromatography
Synonym: LPS
CAS Number: 93572-42-0
EC Number: 297-473-0
MDL Number: MFCD00164401
Product Type: Chemical
Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.
| biological source | Escherichia coli (K-235) |
| color | white to yellow cast |
| form | lyophilized powder |
| impurities | <1% Protein |
| purified by | gel-filtration chromatography |
| Quality Level | 200  |
| shipped in | ambient |
| solubility | water: 4.90-5.10 mg/mL, cloudy to hazy, colorless to faintly yellow |
| storage temp. | 2-8°C |
| Biochem/physiol Actions: | Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics. |
| General description: | This product is extracted from E. coli serotype K235 and purified by gel filtration. The source strain is ATCC 13027. |
| Other Notes: | To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page. |
| Packaging: | 5, 25 mg in glass bottle |
| Preparation Note: | The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution. |
| Symbol |  GHS06 |
| Signal word | Danger |
| Hazard statements | H300 |
| Precautionary statements | P264 - P270 - P301 + P310 - P405 - P501 |
| Hazard Codes | Xn |
| Risk Statements | 20-21-22 |
| Safety Statements | 36 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352201 |