Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane
SIGMA/L2020 - 1-2 mg/mL in Tris-buffered saline, 0.2 μm filtered, BioReagent, suitable for cell culture
Synonym: Laminin
CAS Number: 114956-81-9
MDL Number: MFCD00081739
Product Type: Chemical
Laminin detailed illustration
Bioassay EC50 is defined as the effective concentration of Laminin that elicits a 50% attachment factor in cell based bioassay.
| binding specificity | Peptide Source: Collagen |
| biological source | mouse (Engelbreth-Holm-Swarm mouse sarcoma basement membrane) |
| concentration | 1-2 mg/mL in Tris-buffered saline |
| form | aqueous solution |
| impurities | Microbial Contamination, passes test |
| mol wt | A subunit 400 kDa |
| B1 subunit 210 kDa | |
| B2 subunit 200 kDa | |
| NCBI accession no. |   |
| packaging | pkg of 1 mg |
| product line | BioReagent |
| Quality Level | 300 |
| shipped in | dry ice |
| storage temp. | −20°C |
| surface coverage | 1‑2 μg/cm2 |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P02468 |
| P02469 |
|
| P19137 |
|
| Q61092 |
| Application: | Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane has been used in: • insulin-producing cell (IPC) differentiation. • Infection inhibition assays. • Proliferation Assay. |
| Application: | Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives. |
| Biochem/physiol Actions: | Laminin proteins are integral components of structural scaffolding in animal tissues. They associate with type IV collagen via entactin and perlecan and bind to cell membranes through integrin receptors, dystroglycan glycoprotein complexes and Lutheran blood group glycoproteins. Laminin has active domains for collagen binding, cell adhesion, heparin binding, and neurite outgrowth fragment. |
| Disclaimer: | It is recommended to store this product in working aliquots at -20°C. |
| General description: | Laminins are non-collagenous adhesive glycoproteins with a substantial molecular weight. They are heterotrimeric proteins with three disulfide-linked polypeptides known as the α, β, and γ chains. The multiple polypeptide domains of laminins can bind to multiple cell surface receptors, including several integrin dimers, dystroglycan and syndecans, and other ECM components. This extracellular matrix multidomain trimeric glycoprotein is the main component of basal lamina that supports adhesion, proliferation and differentiation. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research |
| Other Notes: | Laminin is an extracellular matrix multidomain trimeric glycoprotein, and is the main non-collagenous component of basal lamina that supports adhesion, proliferation and differentiation. Laminin is composed of both A, B1 and B2 chains, which are connected by many disulfide bonds. This laminin product was isolated from mouse Engelbreth-Holm-Swarm tumor. |
| Preparation Note: | This product is supplied at a concentration of 1 − 2 mg/mL in 50 mM Tris-HCl, with 150 mM NaCl. It is filtered using a 0.2μm filter. Thaw this solution slowly before use at 2-8°C to avoid gel formation. For use as a coating, dilute in a balanced salt solution, coat culture surface with a minimal volume and incubate at 37°C for two hours. Wash 3 times with PBS before plating cells. Laminin coatings can be stored for one month at 2-8°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |