Leupeptin trifluoroacetate salt
SIGMA/L2023 - ≥90% (HPLC), microbial
Synonym: Acetyl-
CAS Number: 147385-61-3
Empirical Formula (Hill Notation): C20H38N6O4 · xC2HF3O2
Molecular Weight: 426.55 (free base basis)
MDL Number: MFCD00133458
Linear Formula: C20H38N6O4 · xC2HF3O2
Product Type: Chemical
| assay | ≥90% (HPLC) |
| biological source | microbial |
| form | powder |
| InChI | 1S/C20H38N6O4.C2HF3O2/c1- |
| InChI key | WUHGBZVQELGOMH-FRKSIBALSA |
| Quality Level | 200  |
| SMILES string | OC(=O)C(F)(F)F.CC(C)C[C@H |
| solubility | H2O: 10 mM (Solutions are stable only a few hours. Stock solutions are stable up to 6 months at −20 °C.) |
| storage temp. | −20°C |
| Analysis Note: | Leupeptin gives multiple peaks on HPLC because of the formation of tautomeric isomers in solution. Majority of contaminating peptide is racemized leupeptin. |
| Biochem/physiol Actions: | Inhibitor of serine and cysteine proteases. Inhibits plasmin, trypsin, papain, calpain, and cathepsin B. Does not inhibit pepsin, cathepsins A and D, thrombin, or α-chymotrypsin. Effective concentration 10-100 μM. |
| Biochem/physiol Actions: | Inhibitor of serine and cysteine proteases. Inhibits plasmin, trypsin, papain, calpain, and cathepsin B. Does not inhibit pepsin, cathepsins A and D, thrombin, or α-chymotrypsin. Effective concentration 10-100 μM. There have been numerous studies using leupeptin to protect against hearing loss caused by acoustic overstimulation or the ototoxic antibiotic gentamicin. (Loss of cochlear hair cells is believed to be mediated by calpain.) |
| Packaging: | 5, 10, 25, 50, 100 mg in glass bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |