Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)
SIGMA/L2140 - Isolectin B4 (BSI-B4), biotin conjugate, lyophilized powder
Synonym: Bandeirea simplicifolia agglutinin; BS-I
Product Type: Chemical
Agglutination assay: Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)
(Cat. No. L2140) at a concentration of ~1mg solid/mL was serially diluted
(2-fold, top to bottom of plate) and incubated with human erythrocytes type:
B (4% v/v RBC) to calculate Minimum agglutination titer (MAT). Agglutination
is defined as the inability of cells to form a tight button. PBS was used as
blank.
(Cat. No. L2140) at a concentration of ~1mg solid/mL was serially diluted
(2-fold, top to bottom of plate) and incubated with human erythrocytes type:
B (4% v/v RBC) to calculate Minimum agglutination titer (MAT). Agglutination
is defined as the inability of cells to form a tight button. PBS was used as
blank.
Immunofluorescence A transverse section of a Rat lumbar dorsal root ganglion stained with fluorescent markers to distinguish subpopulations of sensory neurons with distinct roles in the pain pathway. Large myelinated neurons and associated fibers are immunoreactive for neurofilament-200 (NF200, in green), while small neurons are divided into peptidergic (in red) and non-peptidercgic (in blue) nociceptors expressing calcitonin gene-related peptide (CGRP) or isolectin B4 (IB4), respectively. Small neurons can encode the intensity of a painful stimulus into firing frequencies, while large neurons are thought to principally mediate sensations of touch, pressure and proprioception. Submitted by Dr C. Tsantoulas, University of Cambridge
| biological source | Bandieraea simplicifolia |
| composition | Protein, ~95% E |
| conjugate | biotin conjugate |
| extent of labeling | 3-6 mol biotin per mol protein |
| form | lyophilized powder |
| potency | <50 μg per mL agglutination activity (using human blood group B erythrocytes) |
| Quality Level | 200  |
| storage temp. | −20°C |
| Analysis Note: | Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C. |
| Application: | Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) has been used for immunohistochemistry and immunofluorescence staining. |
| Biochem/physiol Actions: | BS-I has a major affinity for terminal α- |
| Biochem/physiol Actions: | Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) agglutinates human blood group A and B cells. It is specific for αGalNAc- and αGal. It acts as a reagent for the detection of this αGal epitope in biological materials. Lectin from Bandeiraea simplicifolia is used as a marker for xenoreactive antigen (αGal1−3Gal) and cancer. Lectin plays an important role in fertilization, embryogenesis, inflammation, metastasis and host-parasite recognition. |
| General description: | Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) has A and B subunits, which recognizes αGalNAc and αGal-end groups respectively. Lectins are extracted from the seeds of the legume shrub Griffonia simplicifolia, which are carbohydrate binding proteins. |
| Other Notes: | BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4. BSI-B4 is blood group B specific and has an exclusive affinity for terminal α- |
| Packaging: | 0.2, 1 mg in serum bottle |
| Physical form: | Contains citrate buffer salts and calcium chloride |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |