Anti-Luciferase antibody, Mouse monoclonal
SIGMA/L2164 - clone LUC-1, purified from hybridoma cell culture
Synonym: Anti-Luciferase Antibody
MDL Number: MFCD01092407
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-Luciferase antibody, Mouse Monoclonal, Clone: LUC-1 (Cat. No. L2164) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing Luciferase (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917) and a chemiluminescent substrate. Antibody dilution: 1 μg/mL.
Immunofluorescence 293T cells expressing Luciferase were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 20 μg/mL Anti-Luciferase antibody, Mouse monoclonal, Clone: LUC-1 (Cat. No. L2164) followed by 1:100 Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat (Cat. No. F5262).
Western Blotting Whole extract of 293T cells expressing Luciferase were separated on SDS-PAGE and probed with 1 μg/mL Anti-Luciferase antibody, Mouse monoclonal, Clone: LUC-1 (Cat. No. L2164). The antibody was developed with 1:10,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 293T transfected with Luciferase 2. Negative Control 3. 293T untransfected
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | LUC-1, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | 60 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: 20-40 μg/mL using transfected 293Tcells expressing luciferase |
| immunocytochemistry: suitable | |
| western blot: 2-4 μg/mL using whole extract of transfected 293T cells expressing luciferase | |
| western blot: suitable |
| Application: | Anti-Luciferase antibody, Mouse monoclonal has been used in: • immunoblotting • immunocytochemistry • immunohistochemistry |
| Biochem/physiol Actions: | Anti-Luciferase antibody, Mouse monoclonal recognizes recombinant luciferase in transfected eukaryotic (293T) cells. |
| Biochem/physiol Actions: | Luciferase catalyzes a bioluminescent reaction in the presence of substrate luciferin as well as Mg2+, oxygen and ATP. The luciferase assay is rapid, sensitive, and unlike the CAT assay, does not require a radioactive substrate. Anti-Luciferase antibody can detect the luciferase enzyme expression in situ, providing a means to study the cellular localization of the signal sequence activation. |
| Disclaimer: | Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Luciferase antibody, Mouse monoclonal , (mouse IgG1 isotype) is derived from the LUC-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with firefly (Photinus pyralis) luciferase. Luciferase, a reporter protein contains two binding pockets, one pocket for ATP and other one for luciferin substrate. Both the pockets are in close proximity to each other. |
| General description: | The mouse monoclonal Anti-Luciferase antibody recognizes the recombinant luciferase in transfected cells. This antibody provides user with an alternative detection method for luciferase. Since it directly detects the luciferase protein, it is not dependent on either the luciferase activity or the measeurement of rapid reaction kinetics. This also allows for the detection of the luciferase enzyme expression in situ with reproducibility between sample sets. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in a frost-free freezer, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| Preparation Note: | Working Dilution: 1-2 μg/ml |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |