Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)
SIGMA/L2380 - lyophilized powder
Synonym: Bandeirea simplicifolia agglutinin; BS-I
Product Type: Chemical
Agglutination assay: Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)
(Cat. No. L2380) at a concentration of ~1mg solid/mL was serially diluted
(2-fold, top to bottom of plate) and incubated with human erythrocytes type:
B (4% v/v RBC) to calculate Minimum agglutination titer (MAT). Agglutination
is defined as the inability of cells to form a tight button. PBS was used as
blank.
(Cat. No. L2380) at a concentration of ~1mg solid/mL was serially diluted
(2-fold, top to bottom of plate) and incubated with human erythrocytes type:
B (4% v/v RBC) to calculate Minimum agglutination titer (MAT). Agglutination
is defined as the inability of cells to form a tight button. PBS was used as
blank.
SDS-PAGE Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) (Cat. No. L2380) was assessed on SDS-PAGE.
| composition | Protein, ≥90% biuret |
| form | lyophilized powder |
| impurities | salt, free |
| potency | <20 μg per mL agglutination activity (using human blood group B erythrocytes) |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| Analysis Note: | Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C. |
| Biochem/physiol Actions: | BS-I has a major affinity for terminal α- |
| Other Notes: | BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4. BSI-B4 is blood group B specific and has an exclusive affinity for terminal α- |
| Packaging: | 1, 5 mg in glass bottle |
| Preparation Note: | Purified by affinity chromatography using the method of Hayes, C. E., and Goldstein, I. J., J. Biol. Chem., 249, 1904 (1974). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |