Lipopolysaccharides from Salmonella typhosa
SIGMA/L2387 - purified by gel-filtration chromatography
Synonym: LPS
MDL Number: MFCD00131520
Product Type: Chemical
Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.
| biological source | bacterial (Salmonella typhosa) |
| color | white to faint yellow |
| form | lyophilized powder |
| impurities | <1% Protein |
| purified by | gel-filtration chromatography |
| Quality Level | 200  |
| solubility | water: soluble |
| storage temp. | 2-8°C |
| Application: | Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs). |
| Biochem/physiol Actions: | Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics. |
| General description: | This product is extracted from Salmonella typhosa and purified by gel filtration. The source strain is ATCC 10749. |
| Other Notes: | To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page. |
| Preparation Note: | The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution. |
| Symbol |  GHS06 |
| Signal word | Danger |
| Hazard statements | H300 |
| Precautionary statements | P264 - P270 - P301 + P310 - P405 - P501 |
| RIDADR | UN3373 - class 6.2 Biological substance, Category B |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352201 |