L-Lactic Dehydrogenase from rabbit muscle
SIGMA/L2500 - Type II, ammonium sulfate suspension, 800-1,200 units/mg protein
Synonym: Lactate; (S)-Lactate: NAD+ oxidoreductase; L-LDH; LAD; LD
CAS Number: 9001-60-9
EC Number: 232-617-8
MDL Number: MFCD00131466
Product Type: Chemical
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| foreign activity | pyruvate kinase, myokinase, malic dehydrogenase, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase and α-glycerophosphate dehydrogenase ≤0.01% |
| form | ammonium sulfate suspension |
| mol wt | 140 kDa |
| Quality Level | 200  |
| shipped in | wet ice |
| specific activity | 800-1,200 units/mg protein |
| storage temp. | 2-8°C |
| type | Type II |
| Analysis Note: | Protein determined by biuret. |
| Application: | • for protein-binding measurement • to detect the action of oxaloacetate decarboxylase • to determine serum |
| Biochem/physiol Actions: | Also catalyzes the oxidation of other |
| Biochem/physiol Actions: | Lactic dehydrogenase is responsible for the conversion of pyruvate to lactate in the fermentative metabolism. |
| General description: | Lactic Dehydrogenase (LDH) has a total molecular weight of 140 kDa and is composed of 4 subunits which are designated M subunit (muscle) and H subunit (heart). These subunits may be mixed in any of 5 combinations (M4, M3H1, M2H2, MH3, and H4). Skeletal muscle contains LDH that is predominately M4 with some small amounts of M3H and traces of H2H2. The H and M subunits are quite similar in molecular weight, but differ substantially in amino acid composition. Rabbit muscle LDH dissociates into dimeric species (MW = ~70 kDa) in acetate-chloride at pH 5.0, the dissociation is reversible. Biochemistry, 13, 3527-3531 (1974). Oxidizes glyoxylate and lactate. Isoelectric point: 8.4-8.6 Optimal pH : 7.5 . |
| Other Notes: | One unit will reduce 1.0 μmole of pyruvate to |
| Physical form: | Crystalline suspension in 3.2 M (NH4)2SO4 solution, pH 6.0 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 800-1,200 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.1.1.27 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |