Lectin from Phaseolus vulgaris (red kidney bean)
SIGMA/L2646 - Phytohemagglutinin PHA-M, lyophilized powder
Synonym: Phaseolus vulgaris agglutinin; PHA
EC Number: 232-718-7
Product Type: Chemical
Agglutination assay: Lectin from Phaseolus vulgaris (red kidney bean) (Cat. No. L2646) at a concentration of ~1mg solid/mL was serially diluted (2-fold, top to bottom of plate) and incubated with human erythrocytes type: A (4% v/v RBC) to calculate Minimum agglutination titer (MAT). Agglutination is defined as the inability of cells to form a tight button. PBS was used as blank.
SDS-PAGE Lectin from Phaseolus vulgaris (red kidney bean) (Cat. No. L2646) was assessed on SDS-PAGE.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L2646-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L2646-25MG
| composition | Protein, 50-70% biuret |
| form | lyophilized powder |
| impurities | salt, free |
| potency | <40 μg per mL agglutination activity |
| Quality Level | 300  |
| storage temp. | 2-8°C |
| Analysis Note: | Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C. |
| Biochem/physiol Actions: | PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity. PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins. |
| Packaging: | 10 mg in poly bottle |
| Packaging: | 25, 100 mg in glass bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |