Lectin from Phaseolus vulgaris (red kidney bean)
SIGMA/L2769 - Leucoagglutinin PHA-L, lyophilized powder
Synonym: Phaseolus vulgaris agglutinin; PHA
Product Type: Chemical
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| biological source | Phaseolus vulgaris |
| composition | Protein, ≥60% biuret |
| form | lyophilized powder |
| impurities | salt, free |
| potency | erythroagglutination activity (Does not agglutinate a 2% suspension of human erythrocytes at <62.5 μg lectin/ml.) |
| ≤1.0 μg/mL leucoagglutination activity | |
| ≤5 μg/mL mitogenic activity | |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| Analysis Note: | Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of either human erythrocytes (2% in phosphate buffered saline, pH 6.8) or human leukocytes (107 per mL in saline) after 1 hr incubation at 25 °C. |
| Application: | Lectin from Phaseolus vulgaris (red kidney bean) has been used: • as a positive control in ELISPOT (enzyme-linked immunospot) assay • to induce Th2 polarization for T-cell differentiation in vitro • as controls in the lymphocyte proliferation assays |
| Biochem/physiol Actions: | PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity. PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins. |
| General description: | Phytohemagglutinin (PHA) derived from Phaseolus vulgaris is a tetrameric glycoprotein. It consists of two different subunits, that weighs 120 kDa. |
| Packaging: | 2, 5, 10 mg in glass bottle |
| Preparation Note: | Purified by a modification of the method of Leavitt. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |