Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)
SIGMA/L2895 - Isolectin B4 (BSI-B4), FITC conjugate, lyophilized powder
Synonym: Bandeirea simplicifolia agglutinin; BS-I
Product Type: Chemical
Agglutination assay: Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) (Cat. No. L2895) at a concentration of ~1mg solid/mL was serially diluted (2-fold, top to bottom of plate) and incubated with human erythrocytes type: B (4% v/v RBC) to calculate Minimum agglutination titer (MAT). Agglutination is defined as the inability of cells to form a tight button. PBS was used as blank.
| composition | Protein, ≥70% Lowry (, balance sodium citrate) |
| conjugate | FITC conjugate |
| extent of labeling | ≥1 mol FITC per mol lectin |
| form | lyophilized powder |
| potency | <50 μg per mL agglutination activity (using human blood group B erythrocytes) |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| Analysis Note: | Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C. |
| Application: | Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) has been used for microglia detection in murine astrocytes culture by staining the culture with lectin BS1-B4. It has also been used in the preparation of staining solution to incubate the tissue sections. |
| Biochem/physiol Actions: | BS-I has a major affinity for terminal α- |
| Biochem/physiol Actions: | Lectin is known to be useful in glycoconjugate characterizing, imaging and targeting. Its use in a microarray assay, enable efficient glycome profiling, because of its specific interaction with oligosaccharides, glycoproteins and glycolipids. In plants and fungi, lectin defends against pathogens/feeders. Lectin participates in host recognition and tissue adhesion, thereby aids in the pathogenesis of microorganism. |
| General description: | Lectins are carbohydrate-binding proteins, omnipresent, found in fungi, plants and animals. The structure of lectin is diversely studied in plants and animals. The secondary structure of this protein is rich in β-strands and possesses carbohydrate binding sites on the surface. |
| Other Notes: | BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4. BSI-B4 is blood group B specific and has an exclusive affinity for terminal α- |
| Packaging: | 0.2, 1 mg in serum bottle |
| Packaging: | Package size based on protein content by Lowry. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |