Lysostaphin from Staphylococcus staphylolyticus
SIGMA/L2898 - aseptically filled
Synonym: Glycyl-glycine Endopeptidase
CAS Number: 9011-93-2
MDL Number: MFCD00131556
Product Type: Chemical
Lysostaphin has endopeptidase activity, which cleaves polyglycine cross-links in the cellular wall of Staphylococcus species. Under certain conditions preparations may also exhibit hexosaminidase activity (specific for the glucosaminyl-muramic acid bond of the bacterial carbohydrate backbone, but does not react with S. aureus) and amidase activity.
| antibiotic activity spectrum | Gram-positive bacteria |
| composition | Protein, 40-70% biuret |
| form | powder |
| mode of action | cell wall synthesis | interferes |
| mol wt | 25 kDa |
| Quality Level | 200  |
| specific activity | >500 units/mg protein |
| sterility | aseptically filled |
| storage temp. | −20°C |
| Biochem/physiol Actions: | Lysostaphin exhibit a lytic action against Staphylococcus aureus. It possesses several functions of three major enzymes, such as, glycylglycine endopeptidase, endo-β-N-acetyl glucosamidase and N-acteyl muramyl- |
| Biochem/physiol Actions: | Lysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic. pH Optimum for activity: ~7.5 |
| General description: | Chemical structure: peptide |
| General description: | Lysostaphin is mainly liberated as a proenzyme. It is highly stable at pH 4 and temperature 5°C. |
| Other Notes: | One unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37 °C in a 6.0 ml reaction mixture. |
| Preparation Note: | Prepared from L 7386 |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: >500 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.24.75 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |