Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)
SIGMA/L3019 - Isolectin B4 (BSI-B4), lyophilized powder
Synonym: Bandeirea simplicifolia agglutinin; BS-I
Product Type: Chemical
Agglutination
assay: Lectin from Bandeiraea simplicifolia (Griffonia
simplicifolia) (Cat. No. L3019) at a concentration of ~1mg solid/mL was
serially diluted (2-fold, top to bottom of plate) and incubated with human
erythrocytes type: B (4% v/v RBC) to calculate Minimum agglutination titer
(MAT). Agglutination is defined as the inability of cells to form a tight
button. PBS was used as blank.
assay: Lectin from Bandeiraea simplicifolia (Griffonia
simplicifolia) (Cat. No. L3019) at a concentration of ~1mg solid/mL was
serially diluted (2-fold, top to bottom of plate) and incubated with human
erythrocytes type: B (4% v/v RBC) to calculate Minimum agglutination titer
(MAT). Agglutination is defined as the inability of cells to form a tight
button. PBS was used as blank.
| composition | Protein, ~95% E |
| form | lyophilized powder |
| potency | ≤8 μg per mL agglutination activity (using human blood group B erythrocytes) |
| ≥125 μg per mL agglutination activity (using human blood group A erythrocytes) | |
| Quality Level | 300  |
| storage temp. | −20°C |
| Analysis Note: | Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C. |
| Analysis Note: | Highly purified. |
| Biochem/physiol Actions: | BS-I has a major affinity for terminal α- |
| Other Notes: | BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4. BSI-B4 is blood group B specific and has an exclusive affinity for terminal α- |
| Physical form: | Contains sodium citrate |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |