Escort™ III Transfection Reagent
SIGMA/L3037 - Lipid reagent for transfecting sensitive and primary cells
Product Type: Chemical
Representative product in the bottle
Representative product in the bag
| concentration | 1 mg/mL |
| form | liquid (aqueous solution) |
| grade | Molecular Biology |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| technique(s) | transfection: suitable |
| usage | 1 mL sufficient for 250-1000 transfections |
| Application: | Suitable for transient and stable transfection of nucleic acids into cultured eukaryotic cells. Use approximately 2-8 μl Escort™ III and 2 μg DNA per 6 cm cell culture plate. Protocol optimization provides very efficient transfection. The following cells have been successfully transfected using Escort™ III: A549 C2C12 myotubes Cardiomyocytes (rat) COS-7 Fibroblasts (rat) Germ cells (male rat) Hepatocytes (rat and hamster) HepG2 HeLa Jurkat Keratinocytes (human) Myoblasts (mouse and quail) Myocytes (mouse) NIH3T3 PC-12 Retinal Neurons (rat) Tracheobronchial cells (sheep) |
| Biochem/physiol Actions: | A stable complex is formed when Escort™ III is mixed with DNA in the absence of serum. The complexes are stable and can be directly added to the cell culture medium, where they fuse with the cell membrane, releasing the DNA into the cytoplasm. Note: complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum is without consequence. |
| Disclaimer: | Do not freeze. |
| Features and Benefits: | • Suitable for stable and transient transfection • Optimized for a wide variety of primary cells • Low toxicity • Compatible with both serum and serum-free transfection protocols • Ideal for PC-12 cells |
| General description: | Escort™ III is a unique formulation of a proprietary polycationic lipid and a neutral non-transfecting lipid. This liposome-forming compound is used for transfection of nucleic acids into primary cells. |
| Legal Information: | Escort is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | Escort™ III formulation: 1 mg/mL total lipid in water Note the identity of the lipids used in Escort™ III is confidential. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 41106502 |