Monoclonal Anti-Leptin antibody produced in mouse
SIGMA/L3160 - clone LEP-13, ascites fluid
Synonym: Anti-OB
CAS Number: 93952-18-2
MDL Number: MFCD00803408
Product Type: Chemical
Western Blotting Recombinant Human leptin were separated on SDS-PAGE and probed with Monoclonal Anti-Leptin antibody produced in Mouse, Clone: LEP-13 (Cat. No. L3160). The antibody was developed using 1:10,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. Negative Control antibody 2. 1:500 antibody 3. 1:1,000 antibody
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | LEP-13, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| InChI | 1S/C14H9Cl5/c15-11-5-1-9( |
| InChI key | YVGGHNCTFXOJCH-PGRXLJNUSA |
| isotype | IgG1 |
| mol wt | antigen 16 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| SMILES string | ClC(Cl)(Cl)C(c2c(c(c(c(c2 |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| radioimmunoassay: suitable | |
| western blot: 1:1,000 using recombinant human leptin | |
| UniProt accession no. | P41159  |
| Application: | Monoclonal Anti-Leptin antibody produced in mouse has been used in western blotting. It may be used in radioimmunoassay (RIA) and enzyme linked immunosorbent assay (ELISA). |
| Biochem/physiol Actions: | Leptin is hypothesized to be a ′′satiety factor′′, because the absence of this factor was associated with hyperphagia and obesity. Leptin is involved in appetite regulation. Human obesity is often associated with increased blood leptin levels. Leptin acts through discrete receptors and distant targets to create a feedback loop for body weight regulation. With respect to the target of leptin action, the hypothalamus has received a great deal of attention. The leptin system has also been proposed to contribute to early hematopoiesis. |
| Biochem/physiol Actions: | The antibody reacts specifically with human leptin. By RIA, weak cross-reactivity has been observed with recombinant mouse leptin. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Leptin is a hormone secreted by adipocytes and regulates energy homeostasis, inflammation and neuroendocrine processes, synaptic formation and memory condensation. The physiological effects of leptin are mediated by leptin receptors, ObR and LepR. The receptors are present in brain, lungs, kidneys, liver and adipose tissue. Leptin is the key regulator of obesity and acts directly on neuronal networks to regulate feeding, metabolism, gastric mobility and energy homeostasis. The pathways that mediate the effect of leptin are JAK/STAT, PI3K and JNK. Deficiency of leptin may be a congenital condition and results in thyroid axis abnormalities.. Monoclonal Anti-Leptin reacts specifically with human leptin. A weak cross reactivity with mouse leptin is observed in radioimmunoassay. |
| General description: | Monoclonal Anti-Leptin (OB) (mouse IgG isotype) is derived from the LEP-13 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant human leptin. Leptin (also known as OB, the product of the ob gene) is a 16 kDa, 146 amino acid residue, non-glycosylated polypeptide, secreted by the mature adipocyte. It is strongly expressed in white adipose tissue, and is absent or expressed at extremely low levels in other tissues. The human leptin molecule is translated as a 167 amino acid residue polypeptide, with the first 21 amino acid residues cleaved as a signal peptide. |
| Immunogen: | recombinant human leptin. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |