LB Broth with agar (Luria low salt)
SIGMA/L3272 - Powder microbial growth medium
Synonym: Luria Agar Base (Miller’s Modification)
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L3272-1KG
| application(s) | food and beverages microbiology |
| composition | Agar, 15 g/L |
| NaCl, 0.5 g/L | |
| Tryptone, 10 g/L | |
| Yeast Extract, 5 g/L | |
| form | powder |
| grade | Molecular Biology |
| pH | 6.8-7.2(3.05% solution) |
| Quality Level | 200  |
| sterility | non-sterile |
| storage temp. | room temp |
| suitability | nonselective for Escherichia coli |
| nonselective for coliforms | |
| technique(s) | microbiological culture: suitable |
| Application: | LB Broth with agar (Luria low salt) has been used:• for culturing E. coli DH10B cells. • in the isolation of diazotrophic bacteria. • for transformation of competent E. coli strain Stbl3 for cloning. |
| Application: | Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®. |
| Features and Benefits: | Luria’s low salt LB powder with agar provides: • Easy scale-up using larger package sizes • A budget-friendly alternative to pre-poured plates • Standard formulation |
| General description: | Luria’s low salt LB is a microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. The addition of agar provides a solid medium for microbial growth. |
| Legal Information: | Zeocin is a registered trademark of Cayla Sarl |
| Preparation Note: | 1. Suspend 30.5 g in 1 L of distilled water. 2. Heat to boiling while stirring to dissolve. 3. Autoclave for 15 minutes at 121°C. 4. Cool to 50°C prior to dispensing into petri dishes. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 41106200 |