Escort™ IV Transfection Reagent
SIGMA/L3287 - Lipid reagent for transient and stable transfection of mammalian and insect cells.
Synonym: Gene delivery
Product Type: Chemical
Representative product in the bottle
Image of cos-7 cells transfected with Escort IV
| concentration | 1 mg/mL |
| form | liquid (aqueous solution) |
| grade | Molecular Biology |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| technique(s) | transfection: suitable |
| usage | 1 mL sufficient for 160-500 transfections |
| Application: | Suitable for transient and stable transfection of nucleic acids into cultured eukaryotic cells. Use approximately 4-16 μL Escort™ IV and 2 μg DNA per 6 cm cell culture plate. Protocol optimization provides very efficient transfection. For a list of cells that have been successfully transfected using Escort™ IV, see the Transfection Reagent Selection Guide . |
| Biochem/physiol Actions: | A stable complex is formed when Escort™ IV is mixed with DNA in the absence of serum. The complexes are stable and can be directly added to the cell culture medium, where they fuse with the cell membrane, releasing the DNA into the cytoplasm. Note: complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum is without consequence. |
| Disclaimer: | Do not freeze. |
| Features and Benefits: | • Suitable for stable and transient transfection • Optimized for a wide variety of cell lines • Low toxicity • Compatible with both serum and serum-free transfection protocols • Ideal for Sf9, Sf21 and S2 insect cells |
| General description: | Escort™ IV is a unique formulation of a proprietary polycationic lipid and a neutral non-transfecting lipid. This liposome-forming compound is used for transfection of nucleic acids into a wide variety of eukaryotic cell types. |
| Legal Information: | Escort is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | Escort™ IV formulation: 1 mg/mL total lipid in water Note the identity of the lipids used in Escort™ IV is confidential. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 41106502 |