Anti-Leptin antibody produced in rabbit
SIGMA/L3410 - affinity isolated antibody, buffered aqueous solution
Synonym: Leptin Antibody; Leptin Antibody - Anti-Leptin antibody produced in rabbit; Anti-OB
MDL Number: MFCD00803408
Product Type: Chemical
Western Blotting Human recombinant leptin were separated on SDS-PAGE and probed with Anti-Leptin antibody produced in Rabbit (Cat. No. L3410). The antibody was developed using 1:5,000 Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A9919). Lanes 1. 1:5,000 antibody 2. 1:10,000 antibody 3. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:1,000 using human or mouse recombinant leptin |
| microarray: suitable | |
| western blot: 1:5,000 using human recombinant leptin | |
| UniProt accession no. | P41159  |
| Application: | Anti-Leptin antibody produced in rabbit has been used in: • western blotting • immunohistochemistry • immunocytochemistry • enzyme linked immunosorbent assay (ELISA) |
| Biochem/physiol Actions: | Anti-Leptin reacts specifically with leptin. |
| Biochem/physiol Actions: | Leptin is a circulating cytokine that induces pleiotropic effects on nutrient intake, thermogenesis, metabolism, reproduction and hematopoiesis. Leptin is hypothesized to be a “satiety factor” because the absence of this factor is associated with hyperphagia and obesity in ob/ob mice (homozygous for the obese spontaneous mutation). It appears that leptin is involved in appetite regulation since leptin injection into ob/ob mice reduces their food intake and ultimately their body weight. However, studies have demonstrated that its effects must be more complicated than simple appetite suppression. |
| Biochem/physiol Actions: | Leptin is a hormone secreted by adipocytes and regulates energy homeostasis, inflammation and neuroendocrine processes, synaptic formation and memory condensation. The physiological effects of leptin are mediated by leptin receptors, ObR and LepR. Leptin is the key regulator of obesity and acts directly on neuronal networks to regulate feeding, metabolism and gastric mobility. The pathways that mediate the effect of leptin are Janus kinase (JAK)/signal transducer and activator of transcription (STAT), phosphoinositide 3-kinase (PI3K) and c-Jun N-terminal kinase (JNK). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The gene for leptin (LEP) is mapped to the human chromosome 7q32.1. The encoded protein is a non-glycosylated polypeptide, primarily produced and secreted by the mature adipocytes. Leptin is expressed in white adipose tissue, fetal tissues, skeletal muscle, chondrocytes, gastric chief cells, the placenta, ovary, and many immune cells. It is absent or expressed at extremely low levels in other adult tissues. Human leptin is 85% identical to mouse leptin and 84% identical to rat leptin, while mouse and rat exhibit 96% identity in their leptin molecules. |
| Immunogen: | synthetic peptide corresponds to the amino terminal (amino acid residues 22-40) of human/mouse leptin. |
| Physical form: | Solution in phosphate buffered saline containing 1% bovine serum albumin and 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| Preparation Note: | The antibody is affinity isolated on peptide-agarose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |