Anti-Lamin A−Atto 647N antibody produced in rabbit
SIGMA/L3544 - 1.5-3 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-LAMA; Anti-LMNA; Anti-LNMI; Anti-Lamin A/C
MDL Number: MFCD01633588
Product Type: Chemical
Immunofluorescence Co-staining of nucleolus and nuclear envelope in HeLa cells. HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were co-stained with 1 μg/mL Rabbit Anti-Nucleolin, Atto 488 conjugate (Cat. No. N6288) (nucleolus stained in green) and 10 μg/mL Rabbit Anti-Lamin A, Atto 647N conjugate (Cat. No. L3544) (nuclear envelope stained in red).
Immunofluorescence Co-staining of peroxisomes and nuclear envelop in HeLa cells. HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were co-stained with 20 μg/mL Rabbit Anti-PMP70, Atto 488 Conjugate (Cat. No. P0090) (peroxisomes stained in green) and 10 μg/mL Rabbit Anti-Lamin A, Atto 647N Conjugate (Cat. No. L3544) (nuclear envelope stained in red). Cells were counterstained with DAPI (blue) to stain nuclei.
Immunofluorescence Co-staining of mitochondria and nuclear envelope in HeLa cells. HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were co-stained with 2 μg/mL Mouse Anti-TOM22, Atto 488 Conjugate (Cat. No. T4237) (mitochondria stained in green) and 10 μg/mL Rabbit Anti-Lamin A, Atto 647N Conjugate (Cat. No. L3544) (nuclear envelope stained in red). Cells were counterstained with DAPI (blue) to stain nuclei.
Immunofluorescence HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 5 μg/mL Rabbit Anti-Lamin A, Atto 647N Conjugate (Cat. No. L3544).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1.5-3 mg/mL |
| conjugate | Atto 647N conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct immunofluorescence: 5-10 μg/mL using human HeLa cells |
| UniProt accession no. | P02545  |
| Application: | Anti-Lamin A−Atto 647N antibody produced in rabbit may be used in direct immunofluorescence. |
| Biochem/physiol Actions: | Anti-Lamin A-Atto 647N recognizes human Lamin A. |
| Biochem/physiol Actions: | Lamin A is cleaved into a 47 kDa fragment during apoptosis. Lamin A cleavage seems to be essential for chromatin condensation and nuclear disassembly in apoptosis. Mutations in Lamin A and C have been linked to a variety of rare human diseases including muscular dystrophy, lipodystrophy, cardiomyopathy, neuropathy and progeroid syndromes (collectively termed laminopathies) and to premature aging (Hutchinson-Gilford progeria syndrome). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Lamin A is a structural protein of the nuclear lamina. The nuclear lamina is a meshwork of intermediate filaments that underlies the inner face of the nuclear envelope. The precursor of lamin A, prelamin comprises 98 unique amino acids and also harbors the farnesyl group in C-terminus post-synthesis. The cleavage of the farnesyl group containing end 18 amino acids results in mature Lamin A. Lamin A is mapped to human chromosome 1q21.2. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Protect from prolonged exposure to light. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |